Synthesis of novel lactose-derived oligosaccharides by two-step enzymatic reactions and structural characterization of the oligomers

• Published in: Food research international Vol. 208; p. 116177
• Main Authors: Hu, Hangyu, Hu, Gege, Zhang, Min, Ren, Qingxia, Aziz, Tariq, Sarwar, Abid, Alasmari, Abdullah F., Yang, Zhennai, Yang, Zhang
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01.05.2025
• Subjects: beta-Galactosidase - metabolism; Fermentation; food research; galactose; Galactose - metabolism; galactosides; genes; glucose; glucosides; Inulosucrase; Isomerization; Kluyveromyces - metabolism; Kluyveromyces marxianus var. lactis; Lactobacillus - enzymology; Lactobacillus - genetics; Lactobacillus gasseri; Lactose; Lactose - chemistry; Lactose - metabolism; Oligosaccharides; Oligosaccharides - biosynthesis; Oligosaccharides - chemical synthesis; Oligosaccharides - chemistry; polymerization; Prebiotics; spectroscopy; Sucrose; Sucrose - metabolism; Transfructosylation; β-Galactosidase; Sucrose; DMSO; LS; HH-COSY; DP; TFA; Transfructosylation; MFOS; ESI; β-Galactosidase; HPLC; GC–MS; Isomerization; HMBC; GOS; IS; Oligosaccharides; FOS; NEA; SDS-PAGE; Lactose; NMR; Inulosucrase; IPTG; PMAA; UPLC-MS/MS; GRAS; HSQC; LFOS; RFOS

Exploitation of the added value of lactose by enzymatic synthesis of novel lactose-derived oligosaccharides has been a subject of increasing research interests. In this study, a strategy of two-step enzymatic reactions was designed to first synthesize galacto-oligosaccharide (GOS) from lactose by β-...