Multiple treatment interruptions and protecting HIV-specific CD4 T cells enable durable CD8 T cell response and viral control
Human Immunodeficiency Virus (HIV) remains a global health challenge, and novel approaches to improve HIV control are significantly important. The cell and gene therapy product AGT103-T was previously evaluated (NCT04561258) for safety, immunogenicity, and persistence in seven patients for up to 180...
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Published in | Frontiers in medicine Vol. 11; p. 1342476 |
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Abstract | Human Immunodeficiency Virus (HIV) remains a global health challenge, and novel approaches to improve HIV control are significantly important. The cell and gene therapy product AGT103-T was previously evaluated (NCT04561258) for safety, immunogenicity, and persistence in seven patients for up to 180 days post infusion. In this study, we sought to investigate the impact of AGT103-T treatment upon analytical treatment interruptions (ATIs). Six patients previously infused with AGT103-T were enrolled into an ATI study (NCT05540964), wherein they suspended their antiretroviral therapy (ART) until their viral load reached 100,000 copies/mL in two successive visits, or their CD4 count was reduced to below 300 cells/μL. During the ATI, all patients experienced viral rebound followed by a notable expansion in HIV specific immune responses. The participants demonstrated up to a five-fold increase in total CD8 counts over baseline approximately 1-2 weeks followed by the peak viremia. This coincided with a rise in HIV-specific CD8 T cells, which was attributed to the increase in antigen availability and memory recall. Thus, the protocol was amended to include a second ATI with the first ATI serving as an "auto-vaccination." Four patients participated in a second ATI. During the second ATI, the Gag-specific CD8 T cells were either maintained or rose in response to viral rebound and the peak viremia was substantially decreased. The patients reached a viral set point ranging from 7,000 copies/mL to 25,000 copies/mL. Upon resuming ART, all participants achieved viral control more rapidly than during the first ATI, with CD4 counts remaining within 10% of baseline measurements and without any serious adverse events or evidence of drug resistance. In summary, the rise in CD8 counts and the viral suppression observed in 100% of the study participants are novel observations demonstrating that AGT103-T gene therapy when combined with multiple ATIs, is a safe and effective approach for achieving viral control, with viral setpoints consistently below 25,000 copies/mL and relatively stable CD4 T cell counts. We conclude that HIV cure-oriented cell and gene therapy trials should include ATI and may benefit from designs that include multiple ATIs when induction of CD8 T cells is required to establish viral control. |
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AbstractList | Human Immunodeficiency Virus (HIV) remains a global health challenge, and novel approaches to improve HIV control are significantly important. The cell and gene therapy product AGT103-T was previously evaluated (NCT04561258) for safety, immunogenicity, and persistence in seven patients for up to 180 days post infusion. In this study, we sought to investigate the impact of AGT103-T treatment upon analytical treatment interruptions (ATIs). Six patients previously infused with AGT103-T were enrolled into an ATI study (NCT05540964), wherein they suspended their antiretroviral therapy (ART) until their viral load reached 100,000 copies/mL in two successive visits, or their CD4 count was reduced to below 300 cells/μL. During the ATI, all patients experienced viral rebound followed by a notable expansion in HIV specific immune responses. The participants demonstrated up to a five-fold increase in total CD8 counts over baseline approximately 1-2 weeks followed by the peak viremia. This coincided with a rise in HIV-specific CD8 T cells, which was attributed to the increase in antigen availability and memory recall. Thus, the protocol was amended to include a second ATI with the first ATI serving as an "auto-vaccination." Four patients participated in a second ATI. During the second ATI, the Gag-specific CD8 T cells were either maintained or rose in response to viral rebound and the peak viremia was substantially decreased. The patients reached a viral set point ranging from 7,000 copies/mL to 25,000 copies/mL. Upon resuming ART, all participants achieved viral control more rapidly than during the first ATI, with CD4 counts remaining within 10% of baseline measurements and without any serious adverse events or evidence of drug resistance. In summary, the rise in CD8 counts and the viral suppression observed in 100% of the study participants are novel observations demonstrating that AGT103-T gene therapy when combined with multiple ATIs, is a safe and effective approach for achieving viral control, with viral setpoints consistently below 25,000 copies/mL and relatively stable CD4 T cell counts. We conclude that HIV cure-oriented cell and gene therapy trials should include ATI and may benefit from designs that include multiple ATIs when induction of CD8 T cells is required to establish viral control. |
Author | Mukherjee, Ipsita Kumar, Princy N Liou, Mei-Ling Fledderman, Emily L Canepa, Gaspar E Xiao, Lingzhi Huaman-Vergara, Hellen Jain, Anshika Balogun, Bushirat M Chapoval, Andrei I Conant, Marcus A Galvin, Jeffrey A Boyle, Jefferey S Bordon, José |
AuthorAffiliation | 1 American Gene Technologies International, Inc. , Rockville, MD , United States 3 Washington Health Institute , Washington, DC , United States 2 Division of Infectious Diseases and Tropical Medicine, Georgetown University School of Medicine , Washington, DC , United States |
AuthorAffiliation_xml | – name: 2 Division of Infectious Diseases and Tropical Medicine, Georgetown University School of Medicine , Washington, DC , United States – name: 1 American Gene Technologies International, Inc. , Rockville, MD , United States – name: 3 Washington Health Institute , Washington, DC , United States |
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Cites_doi | 10.1016/j.immuni.2015.08.012 10.1089/aid.2008.0303 10.1001/archinte.163.10.1220 10.1073/pnas.202372199 10.1093/infdis/jiaa270 10.1172/JCI144486 10.1016/S1471-4906(02)02312-8 10.1056/NEJMoa1300662 10.1016/j.omtm.2020.04.024 10.1073/pnas.96.26.15109 10.1128/JVI.01986-20 10.1038/35035103 10.1097/QAI.0000000000001134 10.1038/417095a 10.1038/s41598-018-25943-2 10.1073/pnas.261568398 10.1056/NEJM199905273402114 10.1101/cshperspect.a007054 10.1038/nri3862 10.1038/8400 10.1097/QAD.0000000000001560 10.1093/infdis/jiv022 10.1073/pnas.212518999 10.1074/jbc.M115.662171 10.3389/fmed.2022.1044713 10.1101/cshperspect.a006866 10.7448/IAS.19.1.20697 10.1038/s41598-020-79698-w 10.1016/j.jve.2023.100335 10.1371/journal.ppat.1006792 10.3390/v7082816 |
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Copyright | Copyright © 2024 Jain, Canepa, Liou, Fledderman, Chapoval, Xiao, Mukherjee, Balogun, Huaman-Vergara, Galvin, Kumar, Bordon, Conant and Boyle. Copyright © 2024 Jain, Canepa, Liou, Fledderman, Chapoval, Xiao, Mukherjee, Balogun, Huaman-Vergara, Galvin, Kumar, Bordon, Conant and Boyle. 2024 Jain, Canepa, Liou, Fledderman, Chapoval, Xiao, Mukherjee, Balogun, Huaman-Vergara, Galvin, Kumar, Bordon, Conant and Boyle |
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Keywords | cell and gene therapy (CGT) AGT103-T Human Immunodeficiency Virus (HIV) CCR5 CD4 /CD8 lymphocytes immunotherapy |
Language | English |
License | Copyright © 2024 Jain, Canepa, Liou, Fledderman, Chapoval, Xiao, Mukherjee, Balogun, Huaman-Vergara, Galvin, Kumar, Bordon, Conant and Boyle. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
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Title | Multiple treatment interruptions and protecting HIV-specific CD4 T cells enable durable CD8 T cell response and viral control |
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