Whole-genome analysis of an oxacillin-susceptible CC80 mecA-positive Staphylococcus aureus clinical isolate: insights into the mechanisms of cryptic methicillin resistance

• Published in: Journal of antimicrobial chemotherapy Vol. 70; no. 11; pp. 2956 - 2964
• Main Authors: Sabat, Artur J, Pournaras, Spyros, Akkerboom, Viktoria, Tsakris, Athanassios, Grundmann, Hajo, Friedrich, Alexander W
• Format: Journal Article
• Language: English
• Published: England Oxford Publishing Limited (England) 01.11.2015
• Subjects: Anti-Bacterial Agents - pharmacology; Bacterial Proteins - genetics; Drug resistance; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Genetics; Genome, Bacterial; Genomics; Genotype; Methicillin Resistance; Microbial Sensitivity Tests; Molecular Sequence Data; Molecular Typing; Oxacillin - pharmacology; Penicillin; Penicillin-Binding Proteins - genetics; Plasmids; Sequence Analysis, DNA; Staphylococcal Infections - microbiology; Staphylococcus aureus; Staphylococcus aureus - classification; Staphylococcus aureus - drug effects; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification; Staphylococcus infections

The mec and bla systems, among other genetic factors, are critical in regulating the expression of methicillin resistance in Staphylococcus aureus. We examined by WGS a naturally occurring oxacillin-susceptible mecA-positive S. aureus isolate to identify the mechanism conferring oxacillin susceptibi...