Rate and Equilibrium Constants for Phosphoryltransfer between Active Site Histidines of Escherichia coli HPr and the Signal Transducing Protein IIIGlc

• Published in: The Journal of biological chemistry Vol. 271; no. 52; pp. 33440 - 33445
• Main Authors: Meadow, Norman D., Roseman, Saul
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 27.12.1996; American Society for Biochemistry and Molecular Biology
• Subjects: Antigens, Bacterial - metabolism; Bacterial Proteins - metabolism; Escherichia coli Proteins; Histidine - metabolism; Mutagenesis, Site-Directed; Phosphoenolpyruvate Sugar Phosphotransferase System - metabolism; Phosphorylation; Signal Transduction

The bacterial phosphoenolpyruvate:glycose phosphotransferase system (PTS) plays a central role in catabolizing many sugars; regulation is effected by phosphorylation of PTS proteins. In Escherichia coli, the phosphoryltransfer sequence for glucose uptake is: PEP → Enzyme I(His191) → HPr(His15) → III...