Coupling Sap Flow Velocity and Amino Acid Concentrations as an Alternative Method to 15N Labeling for Quantifying Nitrogen Remobilization by Walnut Trees

• Published in: Plant physiology (Bethesda) Vol. 130; no. 2; pp. 1043 - 1053
• Main Authors: Ela Frak, Millard, Peter, Le Roux, Xavier, Sabine Guillaumie, Wendler, Renate
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Biologists 01.10.2002; American Society of Plant Physiologists
• Subjects: Agronomy. Soil science and plant productions; Amino acids; Arginine; Aspartic Acid; Biological and medical sciences; Biological Transport; Branches; Budbreak; chemical constituents of plants; Citrulline; developmental stages; diurnal variation; Economic plant physiology; Flow velocity; forest trees; Fundamental and applied biological sciences. Psychology; gamma-Aminobutyric Acid; Glutamic Acid; growth & development; isotope labeling; isotopes; Juglans; Juglans nigra; Juglans regia; Light; Metabolism; methods; Nitrogen; Nitrogen Isotopes; Nitrogen metabolism; Nitrogen metabolism and other ones (excepting carbon metabolism); Nutrition. Photosynthesis. Respiration. Metabolism; physiology; plant development; Plant physiology and development; Plant Shoots; Plants; quantitative analysis; Rheology; Sap; Sap flow; seasonal variation; Seasons; shoots; Time Factors; Tree trunks; velocity; Whole Plant and Ecophysiology; woody plants; Xylem; Hybrid variety; Glutamic acid; Trunk; Sap; Xylem; Juglans; Isotope labelling; Nitrogen Isotopes; Arginine; Seasonal variation; Dicotyledones; Angiospermae; Aspartic acid; Chemical concentration; Quantitative analysis; Metabolic storage; Translocation; Root; Tissue specificity; Woody plant; Citrulline; Diurnal variation; Mobilization; Juglandaceae; Aminoacid; GABA; Spermatophyta; Measurement method; Tracers

The temporal dynamics of N remobilization was studied in walnut (Juglans nigra × regia) trees growing in sand culture. Trees were fed with labeled N (15N) during 1999 and unlabeled N in 2000. Total N and 15N contents in different tree compartments were measured during 80 d after bud burst and were u...