Substance P/calcitonin gene‐related peptide and their receptors expression in human periodontal ligament after root canal preparation with five different systems

Aim The purpose of this study was to quantify the effect of five different root canal preparation instruments on Substance P (SP), Calcitonin gene‐related peptide (CGRP) and their receptors expression in healthy human periodontal ligament. Methodology STROBE guidelines were used to design a study us...

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Published inInternational endodontic journal Vol. 57; no. 5; pp. 576 - 585
Main Authors Caviedes‐Bucheli, Javier, Muñoz‐Alvear, Hernan Dario, Lopez‐Moncayo, Luis Fernando, Kacharaju, Kranthi Raja, Velasquez‐Rivera, Adriana Carolina, Carlosama‐Recalde, Luis Alberto, Pazmiño, Juan Camilo, Gomez‐Sosa, Jose Francisco, Diaz‐Barrera, Luis Eduardo, Munoz, Hugo Roberto
Format Journal Article
LanguageEnglish
Published England Wiley Subscription Services, Inc 01.05.2024
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Abstract Aim The purpose of this study was to quantify the effect of five different root canal preparation instruments on Substance P (SP), Calcitonin gene‐related peptide (CGRP) and their receptors expression in healthy human periodontal ligament. Methodology STROBE guidelines were used to design a study using 60 periodontal ligament samples obtained from healthy lower premolars where extraction was indicated for orthodontic reasons. Prior to extraction 40 of these premolars were equally divided into four groups and root canals were prepared using different systems: Mtwo, Reciproc Blue, HyFlex EDM and Plex‐V. Ten premolars were prepared with hand files and served as a positive control group. The remaining 10 premolars where extracted without treatment and served as a negative control group. All periodontal ligament samples were processed to measure the expression of SP, CGRP and their receptors by radioimmunoassay. Kruskal–Wallis and Duncan tests were performed to determine statistically significant differences between the groups for each variable. Results Greater expression of all the peptides measured were found in the hand‐file preparation group, followed by the Reciproc Blue, Mtwo, HyFlex EDM and Plex‐V groups. The lower SP, CGRP and their receptors values were for the intact teeth control group. Kruskal–Wallis test showed statistically significant differences amongst groups (p < .001). Dunn post‐hoc tests showed statistically significant differences in SP, CGRP and their receptors expression between the intact teeth and the hand‐file and Reciproc Blue groups. Hand‐file group showed significant differences with the other groups, except with Reciproc Blue, where no differences were observed in any of the peptides measured. Finally, no differences were observed between Plex‐V and HyFlex in any of the peptides measured. Conclusions Root canal preparation with hand files and Reciproc Blue generates the highest expression of SP, CGRP, NK1 and CGRP1R in human periodontal ligament, whilst Plex‐V and HyFlex maintain the basal expression of neuropeptides and their receptors. Mtwo showed intermediate results between Reciproc Blue and HyFlex.
AbstractList The purpose of this study was to quantify the effect of five different root canal preparation instruments on Substance P (SP), Calcitonin gene-related peptide (CGRP) and their receptors expression in healthy human periodontal ligament. STROBE guidelines were used to design a study using 60 periodontal ligament samples obtained from healthy lower premolars where extraction was indicated for orthodontic reasons. Prior to extraction 40 of these premolars were equally divided into four groups and root canals were prepared using different systems: Mtwo, Reciproc Blue, HyFlex EDM and Plex-V. Ten premolars were prepared with hand files and served as a positive control group. The remaining 10 premolars where extracted without treatment and served as a negative control group. All periodontal ligament samples were processed to measure the expression of SP, CGRP and their receptors by radioimmunoassay. Kruskal-Wallis and Duncan tests were performed to determine statistically significant differences between the groups for each variable. Greater expression of all the peptides measured were found in the hand-file preparation group, followed by the Reciproc Blue, Mtwo, HyFlex EDM and Plex-V groups. The lower SP, CGRP and their receptors values were for the intact teeth control group. Kruskal-Wallis test showed statistically significant differences amongst groups (p < .001). Dunn post-hoc tests showed statistically significant differences in SP, CGRP and their receptors expression between the intact teeth and the hand-file and Reciproc Blue groups. Hand-file group showed significant differences with the other groups, except with Reciproc Blue, where no differences were observed in any of the peptides measured. Finally, no differences were observed between Plex-V and HyFlex in any of the peptides measured. Root canal preparation with hand files and Reciproc Blue generates the highest expression of SP, CGRP, NK1 and CGRP1R in human periodontal ligament, whilst Plex-V and HyFlex maintain the basal expression of neuropeptides and their receptors. Mtwo showed intermediate results between Reciproc Blue and HyFlex.
Aim The purpose of this study was to quantify the effect of five different root canal preparation instruments on Substance P (SP), Calcitonin gene‐related peptide (CGRP) and their receptors expression in healthy human periodontal ligament. Methodology STROBE guidelines were used to design a study using 60 periodontal ligament samples obtained from healthy lower premolars where extraction was indicated for orthodontic reasons. Prior to extraction 40 of these premolars were equally divided into four groups and root canals were prepared using different systems: Mtwo, Reciproc Blue, HyFlex EDM and Plex‐V. Ten premolars were prepared with hand files and served as a positive control group. The remaining 10 premolars where extracted without treatment and served as a negative control group. All periodontal ligament samples were processed to measure the expression of SP, CGRP and their receptors by radioimmunoassay. Kruskal–Wallis and Duncan tests were performed to determine statistically significant differences between the groups for each variable. Results Greater expression of all the peptides measured were found in the hand‐file preparation group, followed by the Reciproc Blue, Mtwo, HyFlex EDM and Plex‐V groups. The lower SP, CGRP and their receptors values were for the intact teeth control group. Kruskal–Wallis test showed statistically significant differences amongst groups (p < .001). Dunn post‐hoc tests showed statistically significant differences in SP, CGRP and their receptors expression between the intact teeth and the hand‐file and Reciproc Blue groups. Hand‐file group showed significant differences with the other groups, except with Reciproc Blue, where no differences were observed in any of the peptides measured. Finally, no differences were observed between Plex‐V and HyFlex in any of the peptides measured. Conclusions Root canal preparation with hand files and Reciproc Blue generates the highest expression of SP, CGRP, NK1 and CGRP1R in human periodontal ligament, whilst Plex‐V and HyFlex maintain the basal expression of neuropeptides and their receptors. Mtwo showed intermediate results between Reciproc Blue and HyFlex.
AimThe purpose of this study was to quantify the effect of five different root canal preparation instruments on Substance P (SP), Calcitonin gene‐related peptide (CGRP) and their receptors expression in healthy human periodontal ligament.MethodologySTROBE guidelines were used to design a study using 60 periodontal ligament samples obtained from healthy lower premolars where extraction was indicated for orthodontic reasons. Prior to extraction 40 of these premolars were equally divided into four groups and root canals were prepared using different systems: Mtwo, Reciproc Blue, HyFlex EDM and Plex‐V. Ten premolars were prepared with hand files and served as a positive control group. The remaining 10 premolars where extracted without treatment and served as a negative control group. All periodontal ligament samples were processed to measure the expression of SP, CGRP and their receptors by radioimmunoassay. Kruskal–Wallis and Duncan tests were performed to determine statistically significant differences between the groups for each variable.ResultsGreater expression of all the peptides measured were found in the hand‐file preparation group, followed by the Reciproc Blue, Mtwo, HyFlex EDM and Plex‐V groups. The lower SP, CGRP and their receptors values were for the intact teeth control group. Kruskal–Wallis test showed statistically significant differences amongst groups (p < .001). Dunn post‐hoc tests showed statistically significant differences in SP, CGRP and their receptors expression between the intact teeth and the hand‐file and Reciproc Blue groups. Hand‐file group showed significant differences with the other groups, except with Reciproc Blue, where no differences were observed in any of the peptides measured. Finally, no differences were observed between Plex‐V and HyFlex in any of the peptides measured.ConclusionsRoot canal preparation with hand files and Reciproc Blue generates the highest expression of SP, CGRP, NK1 and CGRP1R in human periodontal ligament, whilst Plex‐V and HyFlex maintain the basal expression of neuropeptides and their receptors. Mtwo showed intermediate results between Reciproc Blue and HyFlex.
Author Muñoz‐Alvear, Hernan Dario
Lopez‐Moncayo, Luis Fernando
Diaz‐Barrera, Luis Eduardo
Gomez‐Sosa, Jose Francisco
Velasquez‐Rivera, Adriana Carolina
Kacharaju, Kranthi Raja
Munoz, Hugo Roberto
Pazmiño, Juan Camilo
Caviedes‐Bucheli, Javier
Carlosama‐Recalde, Luis Alberto
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  givenname: Luis Eduardo
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  surname: Diaz‐Barrera
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  givenname: Hugo Roberto
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Issue 5
Keywords root canal preparation
NK1 receptor
Substance P
instrumentation systems
calcitonin gene-related peptide
CGRP receptor 1
human periodontal ligament
neurogenic inflammation
Language English
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Snippet Aim The purpose of this study was to quantify the effect of five different root canal preparation instruments on Substance P (SP), Calcitonin gene‐related...
The purpose of this study was to quantify the effect of five different root canal preparation instruments on Substance P (SP), Calcitonin gene-related peptide...
AimThe purpose of this study was to quantify the effect of five different root canal preparation instruments on Substance P (SP), Calcitonin gene‐related...
AIMThe purpose of this study was to quantify the effect of five different root canal preparation instruments on Substance P (SP), Calcitonin gene-related...
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SubjectTerms Calcitonin
Calcitonin gene-related peptide
CGRP receptor 1
Endodontics
human periodontal ligament
instrumentation systems
Ligaments
neurogenic inflammation
Neuropeptides
NK1 receptor
Orthodontics
Peptides
Periodontal ligament
Premolars
Radioimmunoassay
root canal preparation
Root canals
Statistical analysis
Substance P
Teeth
Title Substance P/calcitonin gene‐related peptide and their receptors expression in human periodontal ligament after root canal preparation with five different systems
URI https://onlinelibrary.wiley.com/doi/abs/10.1111%2Fiej.14035
https://www.ncbi.nlm.nih.gov/pubmed/38294105
https://www.proquest.com/docview/3032861579
https://search.proquest.com/docview/2920575374
Volume 57
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