Hydrodynamic chromatography of waxy maize starch

A hydrodynamic column packed with solid beads chemically bonded with N-methyl-D-glucamine residues was used with 90% dimethylsulfoxide (DMSO)-H2O mobile phase as part of a chromatographic system to characterize jet-cooked waxy maize starch. Software calculations based on signals from refractive inde...

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Bibliographic Details
Published inCereal chemistry Vol. 74; no. 6
Main Authors Klavons, J.A, Dintzis, F.R, Millard, M.M
Format Journal Article
LanguageEnglish
Published 01.11.1997
Subjects
ALIMENTOS
ALMIDON DE MAIZ
AMIDON DE MAIS
AMILOPECTINA
AMYLOPECTIN
AMYLOPECTINE
CAPTEUR
CHROMATOGRAPHIE
CHROMATOGRAPHY
CORN STARCH
CROMATOGRAFIA
DETECTORS
FOOD ANALYSIS
FOODS
FRACCIONAMIENTO
FRACTIONATION
FRACTIONNEMENT
MOLECULAR WEIGHT
PESO MOLECULAR
POIDS MOLECULAIRE
PRODUIT ALIMENTAIRE
SENSORES
SENSORS
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Summary:A hydrodynamic column packed with solid beads chemically bonded with N-methyl-D-glucamine residues was used with 90% dimethylsulfoxide (DMSO)-H2O mobile phase as part of a chromatographic system to characterize jet-cooked waxy maize starch. Software calculations based on signals from refractive index and dual-angle light-scattering detectors indicated the column could fractionate molecular weights up to approximately 5 X 10(8). Calculated molecular weight values for the highest molecular weight sample was greatest at the lowest flow rate of 0.1 mL/min. Values of molecular weights and radii of gyration determined by the in-line dual-angle light-scattering detector were significantly less than those determined with an off-line multiangle light-scattering detector that examined samples that had not traveled through the hydrodynamic column. This work has demonstrated the feasibility of using a hydrodynamic column to characterize waxy maize amylopectins. However, considerations of sample shear sensitivity and questions of in-line light scattering detection show that further efforts are required to develop and optimize a chromatographic system to characterize very high molecular weight amylopectins
Bibliography:Q04
1997073404
ISSN:0009-0352
1943-3638
DOI:10.1094/CCHEM.1997.74.6.832