Senescence in cells of the aging and degenerating intervertebral disc: immunolocalization of senescence-associated beta-galactosidase in human and sand rat discs

• Published in: Spine (Philadelphia, Pa. 1976) Vol. 32; no. 3; p. 321
• Main Authors: Gruber, Helen E, Ingram, Jane A, Norton, H James, Hanley, Jr, Edward N
• Format: Journal Article
• Language: English
• Published: United States 01.02.2007
• Subjects: Adolescent; Adult; Aged; Aging - metabolism; Animals; beta-Galactosidase - metabolism; Biomarkers - chemistry; Biomarkers - metabolism; Cellular Senescence - physiology; Female; Gerbillinae; Humans; Immunohistochemistry; Intervertebral Disc - enzymology; Intervertebral Disc - pathology; Male; Middle Aged; Rats; Spinal Diseases - enzymology; Spinal Diseases - pathology

Human intervertebral disc anulus tissue was obtained in a prospective study of cell senescence. Localization of the senescence biomarker beta-galactosidase (senescence associated beta-galactosidase, SA-beta-gal) was used for quantitative determination of the % senescent cells. Discs were obtained fr...