Hepatitis C virus structural proteins and virus-like particles produced in recombinant baculovirus-infected insect cells
Three proteins, namely, the core protein C and envelope glycoproteins E1 and E2, are main structural proteins forming a hepatitis C virus (HCV) virion. The virus structure and assembly and the role of the structural proteins in virion morphogenesis remain unknown because of the lack of an efficient...
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| Published in | Molecular biology (New York) Vol. 44; no. 1; pp. 97 - 108 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
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Dordrecht
SP MAIK Nauka/Interperiodica
01.02.2010
Springer Nature B.V |
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| Abstract | Three proteins, namely, the core protein C and envelope glycoproteins E1 and E2, are main structural proteins forming a hepatitis C virus (HCV) virion. The virus structure and assembly and the role of the structural proteins in virion morphogenesis remain unknown because of the lack of an efficient culture system for HCV to be grown in vitro. Highly efficient heterologous expression systems make it possible to obtain self-assembled, nonreplicating, genome-lacking particles that are morphologically similar to intact virions. Using recombinant baculoviruses expressing the HCV structural protein genes in insect cells, the individual HCV structural proteins were expressed to 25–35% of the total cell protein, and the CE1 and E1E2 heterodimers and HCV-like particles were obtained. It was demonstrated that the recombinant C, E1, and E2 proteins underwent posttranslational modification, the glycoproteins formed a noncovalent heterodimer, and HCV- like particles were located in endoplasmic reticulum membranes of infected cells. The formation of E1E2 dimers and HCV-like particles was used to study the effect of E1 glycosylation on the expression and processing of the coat proteins. |
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| AbstractList | Three proteins, namely, the core protein C and envelope glycoproteins E1 and E2, are main structural proteins forming a hepatitis C virus (HCV) virion. The virus structure and assembly and the role of the structural proteins in virion morphogenesis remain unknown because of the lack of an efficient culture system for HCV to be grown in vitro. Highly efficient heterologous expression systems make it possible to obtain self-assembled, nonreplicating, genome-lacking particles that are morphologically similar to intact virions. Using recombinant baculoviruses expressing the HCV structural protein genes in insect cells, the individual HCV structural proteins were expressed to 25-35% of the total cell protein, and the CE1 and E1E2 heterodimers and HCV-like particles were obtained. It was demonstrated that the recombinant C, E1, and E2 proteins underwent posttranslational modification, the glycoproteins formed a noncovalent heterodimer, and HCV- like particles were located in endoplasmic reticulum membranes of infected cells. The formation of E1E2 dimers and HCV-like particles was used to study the effect of E1 glycosylation on the expression and processing of the coat proteins.[PUBLICATION ABSTRACT] Three proteins, namely, the core protein C and envelope glycoproteins E1 and E2, are main structural proteins forming a hepatitis C virus (HCV) virion. The virus structure and assembly and the role of the structural proteins in virion morphogenesis remain unknown because of the lack of an efficient culture system for HCV to be grown in vitro. Highly efficient heterologous expression systems make it possible to obtain self-assembled, nonreplicating, genome-lacking particles that are morphologically similar to intact virions. Using recombinant baculoviruses expressing the HCV structural protein genes in insect cells, the individual HCV structural proteins were expressed to 25–35% of the total cell protein, and the CE1 and E1E2 heterodimers and HCV-like particles were obtained. It was demonstrated that the recombinant C, E1, and E2 proteins underwent posttranslational modification, the glycoproteins formed a noncovalent heterodimer, and HCV- like particles were located in endoplasmic reticulum membranes of infected cells. The formation of E1E2 dimers and HCV-like particles was used to study the effect of E1 glycosylation on the expression and processing of the coat proteins. |
| Author | Drutza, V. L. Orlova, O. V. Kochetkov, S. N. Koroleva, N. N. Popenko, V. V. Belzhelarskaya, S. N. Rubtzov, P. M. |
| Author_xml | – sequence: 1 givenname: S. N. surname: Belzhelarskaya fullname: Belzhelarskaya, S. N. email: belj@eimb.ru organization: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences – sequence: 2 givenname: N. N. surname: Koroleva fullname: Koroleva, N. N. organization: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences – sequence: 3 givenname: V. V. surname: Popenko fullname: Popenko, V. V. organization: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences – sequence: 4 givenname: V. L. surname: Drutza fullname: Drutza, V. L. organization: Chemical Department, Moscow State University – sequence: 5 givenname: O. V. surname: Orlova fullname: Orlova, O. V. organization: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences – sequence: 6 givenname: P. M. surname: Rubtzov fullname: Rubtzov, P. M. organization: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences – sequence: 7 givenname: S. N. surname: Kochetkov fullname: Kochetkov, S. N. organization: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences |
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| CitedBy_id | crossref_primary_10_1016_j_bbrep_2016_05_019 crossref_primary_10_1021_jm400125h crossref_primary_10_1134_S002689331101002X crossref_primary_10_1134_S0026893312040115 crossref_primary_10_1016_j_pep_2021_105864 crossref_primary_10_1134_S0026893313010123 |
| Cites_doi | 10.1126/science.2523562 10.1016/S0042-6822(03)00141-7 10.1016/S1089-3261(02)00065-X 10.1074/jbc.M602587200 10.1128/JVI.79.13.8400-8409.2005 10.1093/glycob/12.2.95 10.1016/S0168-1656(02)00210-9 10.1006/viro.1996.0164 10.1002/hep.1840360704 10.1128/JVI.70.2.778-786.1996 10.1128/JVI.72.7.6048-6055.1998 10.1128/JVI.71.1.657-662.1997 10.1007/978-94-011-2374-7 10.1128/JVI.72.5.3827-3836.1998 10.5483/BMBRep.2004.37.6.735 10.1128/JVI.68.9.5945-5952.1994 10.1128/JVI.68.8.5063-5073.1994 |
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| Keywords | virus-like particles core-like particles hepatitis C virus HCV structural proteins insect cells glycoprotein glycosylation recombinant baculovirus |
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| References | Hoofnagle (CR1) 2002; 35 Li, Takeda, Kato, Nilsson, Xing, Haag, Cheng, Miyamura (CR11) 2003; 311 Lin, Lindenbach, Pragai, McCourt, Rice (CR5) 1994; 68 Liu, Tackney, Bhat, Prince, Zhang (CR7) 1997; 71 Vauloup-Fellous, Pene, Garaud-Aunis, Harper, Bardin, Suire, Pichard, Schmitt, Sogni, Pierron, Briand, Rosenberg (CR3) 2006; 281 Baumert, Ito, Wong, Liang (CR13) 1998; 72 CR17 Matsumoto, Hwang, Jeng, Zhu, Lai (CR8) 1996; 218 Choo, Kuo, Weiner, Overby, Bradley, Houghton (CR9) 1989; 244 Belzhelarskaya (CR15) 2002; 36 Maniatis, Fritsch, Sambrook (CR16) 1982 Choi, Kim, Park, Kim, Hwang (CR21) 2004; 37 Duvet, Op, Cocquerel, Wychowski, Cacan, Dubuisson (CR25) 2002; 122 Goffard, Callens, Bartosch, Wychowski, Cosset, Montpellier, Dubuisson (CR23) 2005; 79 Reed, Rice (CR14) 2000; 242 Mokhonov, Novikov, Samokhvalov, Shatalov, Selivanov, Prilipov, ’ (CR18) 2002; 47 Yasui, Wakita, Tsukiyama-Kohara, Funahashi, Ichikawa, Kajita, Moradpour, Wands, Kohara (CR6) 1998; 72 Pawlotsky (CR2) 2003; 7 Zhao, Liao, Jiang, Jiang (CR22) 2003; 10 Crawford, Labbé, Cohen, Burroughs, Zhou, Estes (CR10) 1994; 68 King, Possee (CR19) 1992 Dubuisson, Rice (CR4) 1996; 70 Cruz, Maranga, Carrondo (CR12) 2002; 99 Ivanov, Kuzyakin, Kochetkov (CR24) 2005; 45 Drutsa, Kaberdin, Koroleva, Shilov (CR20) 1991; 17 C. Lin (6115_CR5) 1994; 68 A. Goffard (6115_CR23) 2005; 79 J. Dubuisson (6115_CR4) 1996; 70 P.E. Cruz (6115_CR12) 2002; 99 S.E. Crawford (6115_CR10) 1994; 68 M. Matsumoto (6115_CR8) 1996; 218 Q.L. Choo (6115_CR9) 1989; 244 I.L. Drutsa (6115_CR20) 1991; 17 V.V. Mokhonov (6115_CR18) 2002; 47 6115_CR17 J.H. Hoofnagle (6115_CR1) 2002; 35 K. Yasui (6115_CR6) 1998; 72 A.V. Ivanov (6115_CR24) 2005; 45 L.A. King (6115_CR19) 1992 J.M. Pawlotsky (6115_CR2) 2003; 7 S.N. Belzhelarskaya (6115_CR15) 2002; 36 W. Zhao (6115_CR22) 2003; 10 K.E. Reed (6115_CR14) 2000; 242 T.C. Li (6115_CR11) 2003; 311 S. Duvet (6115_CR25) 2002; 122 Q. Liu (6115_CR7) 1997; 71 S.H. Choi (6115_CR21) 2004; 37 T. Maniatis (6115_CR16) 1982 C. Vauloup-Fellous (6115_CR3) 2006; 281 T.F. Baumert (6115_CR13) 1998; 72 |
| References_xml | – volume: 68 start-page: 5063 year: 1994 end-page: 5073 ident: CR5 article-title: Processing of the hepatitis C E2-NS2 region: Identification of the p7 and two distinct E2-specific products with different C termini publication-title: J. Virol. contributor: fullname: Rice – volume: 70 start-page: 778 year: 1996 end-page: 786 ident: CR4 article-title: Hepatitis C virus glycoprotein folding: Disulfide bond formation and association with calnexin publication-title: J. Virology. contributor: fullname: Rice – volume: 68 start-page: 5945 year: 1994 end-page: 5952 ident: CR10 article-title: Characterization of virus-like particles produced by the expression of rotavirus capsid proteins in insect cells publication-title: J. Virol. contributor: fullname: Estes – volume: 244 start-page: 359 year: 1989 end-page: 362 ident: CR9 article-title: Isolation of a cDNA clone derived from blood-borne non-A, non-B viral hepatitis genome publication-title: Science. doi: 10.1126/science.2523562 contributor: fullname: Houghton – volume: 311 start-page: 115 year: 2003 end-page: 124 ident: CR11 article-title: Characterization of self-assembled virus-like particles of human polyomavirus BK generated by recombinant baculoviruses publication-title: Virology. doi: 10.1016/S0042-6822(03)00141-7 contributor: fullname: Miyamura – volume: 7 start-page: 45 year: 2003 end-page: 66 ident: CR2 article-title: Hepatitis C virus genetic variability: Pathogenic and clinical implications publication-title: Clin. Liver Dis. doi: 10.1016/S1089-3261(02)00065-X contributor: fullname: Pawlotsky – volume: 72 start-page: 3827 year: 1998 end-page: 3836 ident: CR13 article-title: Hepatitis C virus structural proteins assemble into virus-like particles in insect cells publication-title: J. Virol. contributor: fullname: Liang – volume: 281 start-page: 27679 year: 2006 end-page: 27692 ident: CR3 article-title: Signal peptide peptidase-catalyzed cleavage of hepatitis C virus core protein is dispensable for virus budding but destabilizes the viral capsid publication-title: J. Biol. Chemistry. doi: 10.1074/jbc.M602587200 contributor: fullname: Rosenberg – volume: 37 start-page: 735 year: 2004 end-page: 740 ident: CR21 article-title: Hepatitis C virus core protein is efficiently released into the culture medium in insect cells publication-title: J. Biochem. Mol. Biol. contributor: fullname: Hwang – volume: 45 start-page: 37 year: 2005 end-page: 86 ident: CR24 article-title: Moleular biology of hepatitis C virus publication-title: Usp. Biokhim. contributor: fullname: Kochetkov – volume: 47 start-page: 9 year: 2002 end-page: 12 ident: CR18 article-title: Genome analysis of Hepatitis C virus strain 274933RU isolated in Russian Federation publication-title: Vopr. Virusol. contributor: fullname: ’ – volume: 71 start-page: 657 year: 1997 end-page: 662 ident: CR7 article-title: Regulated processing of the hepatitis C virus core protein is linked to subcellular localization publication-title: J. Virol. contributor: fullname: Zhang – volume: 79 start-page: 8400 year: 2005 end-page: 8409 ident: CR23 article-title: Role of N-linked glycans in the functions of hepatitis C virus envelope glycoproteins publication-title: J. Virol. doi: 10.1128/JVI.79.13.8400-8409.2005 contributor: fullname: Dubuisson – year: 1992 ident: CR19 publication-title: The Baculovirus Expression System: A Laboratory Guide contributor: fullname: Possee – volume: 122 start-page: 95 year: 2002 end-page: 101 ident: CR25 article-title: Glycosylation of the hepatitis C virus envelope protein E1 occurs posttranslationally in a mannosylphosphoryldolichol-deficient CHO mutant cell line publication-title: Glycobiology. doi: 10.1093/glycob/12.2.95 contributor: fullname: Dubuisson – volume: 99 start-page: 199 year: 2002 end-page: 214 ident: CR12 article-title: Integrated process optimization: lessons from retrovirus and virus-like particle production publication-title: J. Biotechnol. doi: 10.1016/S0168-1656(02)00210-9 contributor: fullname: Carrondo – ident: CR17 – volume: 218 start-page: 43 year: 1996 end-page: 51 ident: CR8 article-title: Homotypic interaction and multimerization of hepatitis C virus core protein publication-title: Virology. doi: 10.1006/viro.1996.0164 contributor: fullname: Lai – volume: 17 start-page: 1487 year: 1991 end-page: 1493 ident: CR20 article-title: Effective method for directed introduction of mutations into plasmids and cloning of single-stranded DNA fragments publication-title: Bioorg. Khimiya. contributor: fullname: Shilov – volume: 35 start-page: 21 year: 2002 end-page: 29 ident: CR1 article-title: Course and outcome of hepatitis C publication-title: Hepatology. doi: 10.1002/hep.1840360704 contributor: fullname: Hoofnagle – volume: 72 start-page: 6048 year: 1998 end-page: 6055 ident: CR6 article-title: The native form and maturation process of the hepatitis C virus core protein publication-title: J. Virol. contributor: fullname: Kohara – volume: 242 start-page: 55 year: 2000 end-page: 84 ident: CR14 article-title: Overview of hepatitis C virus genome structure, polyprotein processing, and protein properties publication-title: Curr. Top. Microbiol. Immunol. contributor: fullname: Rice – volume: 36 start-page: 371 year: 2002 end-page: 385 ident: CR15 article-title: Baculoviral expression system in insect cells publication-title: Mol. Biol. contributor: fullname: Belzhelarskaya – year: 1982 ident: CR16 publication-title: Molecular Cloning: A Laboratory Manual contributor: fullname: Sambrook – volume: 10 start-page: 2226 year: 2003 end-page: 2231 ident: CR22 article-title: No requirement of HCV 5’NCR for HCV-like particles assembly in insect cells publication-title: J. Gastroenterol. contributor: fullname: Jiang – volume: 17 start-page: 1487 year: 1991 ident: 6115_CR20 publication-title: Bioorg. Khimiya. contributor: fullname: I.L. Drutsa – volume: 70 start-page: 778 year: 1996 ident: 6115_CR4 publication-title: J. Virology. doi: 10.1128/JVI.70.2.778-786.1996 contributor: fullname: J. Dubuisson – volume: 72 start-page: 6048 year: 1998 ident: 6115_CR6 publication-title: J. Virol. doi: 10.1128/JVI.72.7.6048-6055.1998 contributor: fullname: K. Yasui – volume: 242 start-page: 55 year: 2000 ident: 6115_CR14 publication-title: Curr. Top. Microbiol. Immunol. contributor: fullname: K.E. Reed – volume: 71 start-page: 657 year: 1997 ident: 6115_CR7 publication-title: J. Virol. doi: 10.1128/JVI.71.1.657-662.1997 contributor: fullname: Q. Liu – volume: 45 start-page: 37 year: 2005 ident: 6115_CR24 publication-title: Usp. Biokhim. contributor: fullname: A.V. Ivanov – volume-title: The Baculovirus Expression System: A Laboratory Guide year: 1992 ident: 6115_CR19 doi: 10.1007/978-94-011-2374-7 contributor: fullname: L.A. King – volume: 122 start-page: 95 year: 2002 ident: 6115_CR25 publication-title: Glycobiology. doi: 10.1093/glycob/12.2.95 contributor: fullname: S. Duvet – volume: 99 start-page: 199 year: 2002 ident: 6115_CR12 publication-title: J. Biotechnol. doi: 10.1016/S0168-1656(02)00210-9 contributor: fullname: P.E. Cruz – volume: 72 start-page: 3827 year: 1998 ident: 6115_CR13 publication-title: J. Virol. doi: 10.1128/JVI.72.5.3827-3836.1998 contributor: fullname: T.F. Baumert – volume: 37 start-page: 735 year: 2004 ident: 6115_CR21 publication-title: J. Biochem. Mol. Biol. doi: 10.5483/BMBRep.2004.37.6.735 contributor: fullname: S.H. Choi – volume: 35 start-page: 21 year: 2002 ident: 6115_CR1 publication-title: Hepatology. doi: 10.1002/hep.1840360704 contributor: fullname: J.H. Hoofnagle – volume: 79 start-page: 8400 year: 2005 ident: 6115_CR23 publication-title: J. Virol. doi: 10.1128/JVI.79.13.8400-8409.2005 contributor: fullname: A. Goffard – ident: 6115_CR17 – volume-title: Molecular Cloning: A Laboratory Manual year: 1982 ident: 6115_CR16 contributor: fullname: T. Maniatis – volume: 281 start-page: 27679 year: 2006 ident: 6115_CR3 publication-title: J. Biol. Chemistry. doi: 10.1074/jbc.M602587200 contributor: fullname: C. Vauloup-Fellous – volume: 68 start-page: 5945 year: 1994 ident: 6115_CR10 publication-title: J. Virol. doi: 10.1128/JVI.68.9.5945-5952.1994 contributor: fullname: S.E. Crawford – volume: 244 start-page: 359 year: 1989 ident: 6115_CR9 publication-title: Science. doi: 10.1126/science.2523562 contributor: fullname: Q.L. Choo – volume: 218 start-page: 43 year: 1996 ident: 6115_CR8 publication-title: Virology. doi: 10.1006/viro.1996.0164 contributor: fullname: M. Matsumoto – volume: 311 start-page: 115 year: 2003 ident: 6115_CR11 publication-title: Virology. doi: 10.1016/S0042-6822(03)00141-7 contributor: fullname: T.C. Li – volume: 36 start-page: 371 year: 2002 ident: 6115_CR15 publication-title: Mol. Biol. contributor: fullname: S.N. Belzhelarskaya – volume: 47 start-page: 9 year: 2002 ident: 6115_CR18 publication-title: Vopr. Virusol. contributor: fullname: V.V. Mokhonov – volume: 68 start-page: 5063 year: 1994 ident: 6115_CR5 publication-title: J. Virol. doi: 10.1128/JVI.68.8.5063-5073.1994 contributor: fullname: C. Lin – volume: 7 start-page: 45 year: 2003 ident: 6115_CR2 publication-title: Clin. Liver Dis. doi: 10.1016/S1089-3261(02)00065-X contributor: fullname: J.M. Pawlotsky – volume: 10 start-page: 2226 year: 2003 ident: 6115_CR22 publication-title: J. Gastroenterol. contributor: fullname: W. Zhao |
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| SubjectTerms | Baculovirus Biochemistry Biomedical and Life Sciences Cell Molecular Biology Gene expression Hepatitis Hepatitis C virus Human Genetics Insects Life Sciences Molecular biology Proteins |
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| Title | Hepatitis C virus structural proteins and virus-like particles produced in recombinant baculovirus-infected insect cells |
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