Cell surface labeling of CFTR in T84 cells

• Published in: The American journal of physiology Vol. 264; no. 2 Pt 1; p. C491
• Main Authors: Prince, L S, Tousson, A, Marchase, R B
• Format: Journal Article
• Language: English
• Published: United States 01.02.1993
• Subjects: Autoradiography; Biotin - analogs & derivatives; Biotin - metabolism; Biotin - pharmacology; Cell Line; Cell Membrane - metabolism; Colforsin - pharmacology; Cystic Fibrosis - metabolism; Cystic Fibrosis Transmembrane Conductance Regulator; Electrophoresis, Polyacrylamide Gel; Humans; Membrane Proteins - metabolism; Periodic Acid - pharmacology; Precipitin Tests

To distinguish cystic fibrosis transmembrane conductance regulator (CFTR) at the surface of epithelial cells from that present in intracellular membranes, intact T84 cells were treated with periodate and biotin-LC-hydrazide to derivatize exposed glycoconjugates. Cell lysates were then passed over a...