Cas4-Cas1 Is a Protospacer Adjacent Motif-Processing Factor Mediating Half-Site Spacer Integration During CRISPR Adaptation

• Published in: CRISPR journal Vol. 4; no. 4; p. 536
• Main Authors: Kieper, Sebastian N, Almendros, Cristóbal, Haagsma, Anna C, Barendregt, Arjan, Heck, Albert J R, Brouns, Stan J J
• Format: Journal Article
• Language: English
• Published: United States 01.08.2021
• Subjects: Binding Sites; CRISPR-Associated Proteins - chemistry; CRISPR-Associated Proteins - genetics; CRISPR-Associated Proteins - metabolism; CRISPR-Cas Systems; DNA, Bacterial; Gene Editing - methods; Gene Order; Multiprotein Complexes; Nucleotide Motifs; Plasmids - chemistry; Plasmids - genetics; Protein Binding; Protein Multimerization

The immunization of bacteria and archaea against invading viruses via CRISPR adaptation is critically reliant on the efficient capture, accurate processing, and integration of CRISPR spacers into the host genome. The adaptation proteins Cas1 and Cas2 are sufficient for successful spacer acquisition...