Purification and immunochemical detection of a quantitatively major collagen in the dermis of sea cucumber Apostichopus japonicus

• Published in: Fisheries science Vol. 88; no. 1; pp. 173 - 180
• Main Authors: Mizuta, Shoshi, Koizumi, Yuuki, Yokoyama, Yoshihiro, Yoshinaka, Reiji
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Japan 2022; Springer Nature B.V
• Subjects: 2-Mercaptoethanol; Acetic acid; Analytical methods; Apostichopus japonicus; Biomedical and Life Sciences; Collagen; Dermis; Digestion; Disaggregation; Electrophoresis; Ethylenediaminetetraacetic acids; Fibers; Fish & Wildlife Biology & Management; Food Science; Freshwater & Marine Ecology; Gel electrophoresis; Gels; Guanidine hydrochloride; Life Sciences; Marine invertebrates; Original Article; Pepsin; Phosphates; Polyacrylamide; Sodium; Sodium chloride; Sodium dodecyl sulfate; Sodium hydroxide; Sodium lauryl sulfate; Urea; Water purification; Sea cucumber; Body wall; Collagen; Echinoderm; Dermis; Protein; Holothurid

Pepsin-solubilized collagen (PSC) was prepared from the dermis of sea cucumber Apostichopus japonicus (green type) by performing pepsin digestion to collagen fiber pretreated with disaggregating solution (0.1 M Tris–HCl, pH 8.0, containing 0.5 M NaCl, 0.05 M ethylenediaminetetraacetic acid (EDTA), a...