The long non-coding RNA Meg3 mediates imprinted gene expression during stem cell differentiation

• Published in: Nucleic acids research Vol. 52; no. 11; pp. 6183 - 6200
• Main Authors: Farhadova, Sabina, Ghousein, Amani, Charon, François, Surcis, Caroline, Gomez-Velazques, Melisa, Roidor, Clara, Di Michele, Flavio, Borensztein, Maud, De Sario, Albertina, Esnault, Cyril, Noordermeer, Daan, Moindrot, Benoit, Feil, Robert
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 24.06.2024
• Subjects: Animals; Biochemistry, Molecular Biology; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - metabolism; Cell Differentiation - genetics; Cellular Biology; DNA Methylation; Gene Expression Regulation, Developmental; Gene regulation, Chromatin and Epigenetics; Genomic Imprinting; Genomics; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - metabolism; Life Sciences; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice; MicroRNAs - genetics; MicroRNAs - metabolism; Mouse Embryonic Stem Cells - cytology; Mouse Embryonic Stem Cells - metabolism; Nuclear Proteins; Pregnancy Proteins; Promoter Regions, Genetic; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Subcellular Processes

The imprinted Dlk1-Dio3 domain comprises the developmental genes Dlk1 and Rtl1, which are silenced on the maternal chromosome in different cell types. On this parental chromosome, the domain's imprinting control region activates a polycistron that produces the lncRNA Meg3 and many miRNAs (Mirg)...