TCA cycle flux estimates from NMR- and GC-MS-determined [13C]glutamate isotopomers in liver

• Published in: The American journal of physiology Vol. 272; no. 6 Pt 1; p. C2049
• Main Authors: Vogt, J A, Yarmush, D M, Yu, Y M, Zupke, C, Fischman, A J, Tompkins, R G, Burke, J F
• Format: Journal Article
• Language: English
• Published: United States 01.06.1997
• Subjects: Acetyl Coenzyme A - metabolism; Animals; Carbon Isotopes; Citric Acid Cycle; Gas Chromatography-Mass Spectrometry; Glutamic Acid - metabolism; Lactates - metabolism; Least-Squares Analysis; Liver - metabolism; Magnetic Resonance Spectroscopy - methods; Models, Biological; Oxaloacetates - metabolism; Pyruvates - metabolism; Rabbits; Reproducibility of Results

Infusion of 13C-labeled lactate into rabbits and the subsequent measurement of glutamate isotopomers by 13C nuclear magnetic resonance (NMR) spectroscopy enables one to calculate relative flow rates associated with the tricarboxylic acid (TCA) cycle, albeit with a lower precision than one would obta...