Binding of DL-[3H]-alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) to rat cortex membranes reveals two sites or affinity states

• Published in: Journal of receptor research Vol. 11; no. 5; p. 727
• Main Authors: Giberti, A, Ratti, E, Gaviraghi, G, van Amsterdam, F T
• Format: Journal Article
• Language: English
• Published: United States 1991
• Subjects: alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid; Animals; Cerebral Cortex - chemistry; Ibotenic Acid - analogs & derivatives; Ibotenic Acid - chemistry; Male; Radioligand Assay - methods; Rats; Rats, Inbred Strains; Receptors, AMPA; Receptors, Neurotransmitter - chemistry; Synaptic Membranes - chemistry; Tritium

A method for measuring [3H]-AMPA binding in rat cortex membranes is described. Specific binding was saturable and accounted for 95% of total binding at 5 nM of [3H]-AMPA. Non linear curve fitting of [3H]-AMPA saturation isotherms suggested the presence of two binding sites: the high affinity site sh...