In Vitro Evaluation of Cytotoxicity and Oxidative Stress Induced by Multiwalled Carbon Nanotubes in Murine RAW 264.7 Macrophages and Human A549 Lung Cells

• Published in: Biomedical and environmental sciences Vol. 24; no. 6; pp. 593 - 601
• Main Authors: CHEN, Bo, LIU, Ying, SONG, Wei Ming, HAYASHI, Yasuhiko, DING, Xun Cheng, LI, Wei Hua
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.12.2011; Key Laboratory of Public Health Safety, Ministry of Education, School of Public Health, Fudan University,Shanghai 200032, China%Putuo District Center for Disease Control and Prevention, Shanghai 200333, China%Graduate School of Engineering, Nagoya Institute of Technology, Nagoya 466-8555, Japan%NPFPC Key Laboratory of Contraceptive & Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032,China
• Subjects: A549 cells; A549细胞; Animals; Cell Culture Techniques; Cell Line; Cell Survival - drug effects; Cytotoxicity; Dose-Response Relationship, Drug; Humans; Lung - drug effects; Lung - enzymology; Lung - metabolism; Lung - pathology; Macrophages, Alveolar - drug effects; Macrophages, Alveolar - enzymology; Macrophages, Alveolar - metabolism; Macrophages, Alveolar - pathology; Mice; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Microscopy, Fluorescence; Multi-wall carbon nanotubes; Nanotubes, Carbon - chemistry; Nanotubes, Carbon - toxicity; Oxidative stress; Oxidative Stress - drug effects; RAW 264.7 cells; Reactive Oxygen Species - metabolism; Surface Properties; 体外细胞毒性; 多壁碳纳米管; 小鼠; 巨噬细胞; 氧化应激反应; 肺癌细胞; 诱导; Oxidative stress; Cytotoxicity; A549 cells; Multi-wall carbon nanotubes; RAW 264.7 cells
• DOI: 10.3967/0895-3988.2011.06.002
• ISSN: 0895-3988

Objective To investigate in vitro cytotoxicity and oxidative stress response induced by multiwalled carbon nanotubes （MWCNTs）. Methods Cultured macrophages （murine RAW264.7 cells） and alveolar epithelium cells type II （human A549 lung cells） were exposed to the blank control, DNA salt control, and the MWCNTs suspensions at 2.5, 10, 25, and 100 ug/mL for 24 h. Each treatment was evaluated by cell viability, cytotoxicity and oxidative stress. Results Overall, both cell lines had similar patterns in response to the cytotoxicity and oxidative stress of MWCNTs. DNA salt treatment showed no change compared to the blank control. In both cell lines, significant changes at the doses of 25 and 100 ug/mL treatments were found in cell viabilities, cytotoxicity, and oxidative stress indexes. The reactive oxygen species （ROS） generation was also found to be significantly higher at the dose of 10 ug/mL treatment, whereas no change was seen in most of the indexes. The ROS generation in both cell lines went up in minutes, reached the climax within an hour and faded down after several hours. Conclusion Exposure to MWCNTs resulted in a dose-dependent cytotoxicity in cultured RAW264.7 cells and A549 cells, that was closely correlated to the increased oxidative stress.