Tandem phage-display for the identification of non-overlapping binding pairs of recombinant affinity reagents

• Published in: Nucleic acids research Vol. 45; no. 18; p. e158
• Main Authors: Gorman, Kevin T, Roby, Lauren C, Giuffre, Allison, Huang, Renhua, Kay, Brian K
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 13.10.2017
• Subjects: Affinity Labels - metabolism; Cell Surface Display Techniques - methods; Enzyme-Linked Immunosorbent Assay; Genetic Techniques; High-Throughput Screening Assays - methods; Humans; Methods Online; Peptide Library; Polymerization; Protein Binding; Protein Interaction Domains and Motifs; Recombinant Proteins - chemistry; Recombinant Proteins - metabolism

The 'sandwich' binding format, which uses two reagents that can bind simultaneously to a given analyte, is the gold standard in diagnostics and many biochemical techniques. One of the bottlenecks in creating a sandwich assay is identifying pairs of reagents that bind non-competitively to t...