Cryopreservation of in vitro-produced ovine embryos

The purpose of this study was to evaluate different cryopreservation protocols for in vitro-produced ovine embryos and assess the survival rate after cryopreservation. The experiment was also designed to examine whether this technique is feasible to apply in large-scale operations. In a first experi...

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Published inSmall ruminant research Vol. 63; no. 3; pp. 288 - 296
Main Authors Martínez, A.G., Valcárcel, A., Furnus, C.C., de Matos, D.G., Iorio, G., de las Heras, M.A.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.06.2006
Subjects
cell nucleus
cryopreservation
cryoprotectants
culture media
embryo (animal)
embryo hatching rate
embryogenesis
embryonic mortality
ethylene glycol
fetal death
glycerol
in vitro fertilization
In vitro survival
Lambing
lambing rate
sheep
sucrose
Transfer
vitrification
Transfer
Lambing
In vitro survival
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Abstract The purpose of this study was to evaluate different cryopreservation protocols for in vitro-produced ovine embryos and assess the survival rate after cryopreservation. The experiment was also designed to examine whether this technique is feasible to apply in large-scale operations. In a first experiment, ovine embryos cryopreserved in ethylene glycol using 3 steps (E-3S) showed a higher hatching rate and nuclei number than freezing with glycerol in 3 steps (G-3S) (40.0% versus 20.0% and 135.4 ± 20.7 versus 118.5 ± 19.5, respectively). In a second experiment, vitrification with ethylene glycol + Ficoll 70 + sucrose (EFS) recorded a higher hatching rate and nuclei numbers than vitrification with propylene glycol + glycerol (Pg + Gly) (51.1% versus 31.1% and 133.8 ± 36.8 versus 113.5 ± 22.0, respectively). In a third phase, vitrification of embryos with ethylene glycol + glycerol (Eg + Gly) resulted in higher development and hatching rates and nuclei number than EFS (87.3% versus 65.4%; 76.4% versus 54.5% and 135.7 ± 34.5 versus 113.1 ± 14.1, respectively). In a fourth experiment, fresh in vivo embryos produced a higher lambing rate (67.8%) than the other methods, and E-3S in vitro resulted in lower lambing rates (23.0%). E-3S in vivo, Eg + Gly in vivo, fresh in vitro and Eg + Gly in vitro recorded similar lambing rates (42.8, 37.5, 37.5 and 26.6%, respectively). Ewes receiving in vitro-produced ovine embryos resulted in higher assisted births and perinatal losses than those receiving in vivo-produced embryos (3.6% versus 16.9% and 1.2% versus 10.1%, respectively). Results demonstrate that the transfer of in vitro-produced embryos vitrified with Eg + Gly could have wide application, if the negative side effects of the produced offspring can be managed.
AbstractList The purpose of this study was to evaluate different cryopreservation protocols for in vitro-produced ovine embryos and assess the survival rate after cryopreservation. The experiment was also designed to examine whether this technique is feasible to apply in large-scale operations. In a first experiment, ovine embryos cryopreserved in ethylene glycol using 3 steps (E-3S) showed a higher hatching rate and nuclei number than freezing with glycerol in 3 steps (G-3S) (40.0% versus 20.0% and 135.4 ± 20.7 versus 118.5 ± 19.5, respectively). In a second experiment, vitrification with ethylene glycol + Ficoll 70 + sucrose (EFS) recorded a higher hatching rate and nuclei numbers than vitrification with propylene glycol + glycerol (Pg + Gly) (51.1% versus 31.1% and 133.8 ± 36.8 versus 113.5 ± 22.0, respectively). In a third phase, vitrification of embryos with ethylene glycol + glycerol (Eg + Gly) resulted in higher development and hatching rates and nuclei number than EFS (87.3% versus 65.4%; 76.4% versus 54.5% and 135.7 ± 34.5 versus 113.1 ± 14.1, respectively). In a fourth experiment, fresh in vivo embryos produced a higher lambing rate (67.8%) than the other methods, and E-3S in vitro resulted in lower lambing rates (23.0%). E-3S in vivo, Eg + Gly in vivo, fresh in vitro and Eg + Gly in vitro recorded similar lambing rates (42.8, 37.5, 37.5 and 26.6%, respectively). Ewes receiving in vitro-produced ovine embryos resulted in higher assisted births and perinatal losses than those receiving in vivo-produced embryos (3.6% versus 16.9% and 1.2% versus 10.1%, respectively). Results demonstrate that the transfer of in vitro-produced embryos vitrified with Eg + Gly could have wide application, if the negative side effects of the produced offspring can be managed.
The purpose of this study was to evaluate different cryopreservation protocols for in vitro-produced ovine embryos and assess the survival rate after cryopreservation. The experiment was also designed to examine whether this technique is feasible to apply in large-scale operations. In a first experiment, ovine embryos cryopreserved in ethylene glycol using 3 steps (E-3S) showed a higher hatching rate and nuclei number than freezing with glycerol in 3 steps (G-3S) (40.0% versus 20.0% and 135.4 ± 20.7 versus 118.5 ± 19.5, respectively). In a second experiment, vitrification with ethylene glycol + Ficoll 70 + sucrose (EFS) recorded a higher hatching rate and nuclei numbers than vitrification with propylene glycol + glycerol (Pg + Gly) (51.1% versus 31.1% and 133.8 ± 36.8 versus 113.5 ± 22.0, respectively). In a third phase, vitrification of embryos with ethylene glycol + glycerol (Eg + Gly) resulted in higher development and hatching rates and nuclei number than EFS (87.3% versus 65.4%; 76.4% versus 54.5% and 135.7 ± 34.5 versus 113.1 ± 14.1, respectively). In a fourth experiment, fresh in vivo embryos produced a higher lambing rate (67.8%) than the other methods, and E-3S in vitro resulted in lower lambing rates (23.0%). E-3S in vivo, Eg + Gly in vivo, fresh in vitro and Eg + Gly in vitro recorded similar lambing rates (42.8, 37.5, 37.5 and 26.6%, respectively). Ewes receiving in vitro-produced ovine embryos resulted in higher assisted births and perinatal losses than those receiving in vivo-produced embryos (3.6% versus 16.9% and 1.2% versus 10.1%, respectively). Results demonstrate that the transfer of in vitro-produced embryos vitrified with Eg + Gly could have wide application, if the negative side effects of the produced offspring can be managed.
Author Furnus, C.C.
Valcárcel, A.
de Matos, D.G.
Martínez, A.G.
Iorio, G.
de las Heras, M.A.
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Cites_doi 10.1016/0093-691X(84)90368-6
10.1016/0011-2240(89)90026-6
10.1095/biolreprod52.3.676
10.1016/0093-691X(94)00020-U
10.1002/mrd.1120160207
10.1093/oxfordjournals.humrep.a135837
10.1016/0011-2240(84)90079-8
10.1111/j.1439-0531.1993.tb00113.x
10.1016/0734-9750(96)00005-5
10.1016/0093-691X(95)00360-K
10.1530/jrf.0.1070175
10.1016/0093-691X(86)90200-1
10.1016/0093-691X(93)90025-Z
10.1016/S0093-691X(97)00378-6
10.1016/S0378-4320(98)00089-X
10.1530/jrf.0.1030033
10.1530/jrf.0.0300493
10.1095/biolreprod53.6.1385
10.1016/0093-691X(92)90395-8
10.1016/0093-691X(95)00417-7
10.1095/biolreprod50.2.390
10.1016/S0093-691X(05)80054-8
10.1016/S0378-4320(97)00034-1
10.1006/cryo.1995.1007
10.1095/biolreprod66.5.1244
10.1002/(SICI)1098-2795(199907)53:3<318::AID-MRD7>3.0.CO;2-O
10.1016/S0378-4320(02)00162-8
10.1006/cryo.1999.2159
10.2527/1999.7782234x
10.1016/0093-691X(91)90289-P
10.1016/S0093-691X(98)00098-3
10.1530/jrf.0.0980139
10.1530/jrf.0.0960187
10.1071/R96079
10.1016/0011-2240(87)90042-3
10.1016/S0011-2240(02)00139-6
10.1016/S0093-691X(00)00293-4
10.1186/BF03548317
10.1016/S0093-691X(99)00198-3
10.4141/cjas96-069
10.1016/0093-691X(95)00122-O
10.1530/jrf.0.0890091
10.1016/0093-691X(95)00327-5
10.1016/0093-691X(86)90143-3
10.1016/0093-691X(87)90266-4
10.1002/mrd.1080340306
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References Thompson, Gardner, Pugh, Mc Millan, Tervit (bib45) 1995; 53
Walker, Hartwich, Seamark (bib47) 1996; 45
Rall (bib36) 1987; 24
Baldassarre, Aste, Argerich, de Matos (bib1) 1992
Cheng, Moor, Polge (bib5) 1986; 25
Mahmoudzadeh, Van Soom, Bols, Ysebaert, de Kruif (bib25) 1995; 103
Parrish, Susko-Parrish, Leibfried-Rutledge, Critser, Eyestone, First (bib32) 1986; 25
Winterberger-Torres, Sevellec (bib48) 1987
Széll, Shelton, Széll (bib41) 1989; 26
Tervit, Gold (bib43) 1984; 21
Songsasen, Buckrell, Plante, Leibo (bib39) 1995; 32
Holm, Walker, Seamark (bib18) 1996; 107
Ledda, Bogliolo, Leon, Naitana (bib22) 1999; 77
Papadopoulos, Rizos, Duffy, Wade, Quinn, Boland, Lonergan (bib31) 2002; 74
Yang, Lu, Gordon, Polge (bib50) 1992; 37
Pollard, Leibo (bib33) 1994; 41
Tervit, Whittingham, Rowson (bib44) 1972; 30
Greve, Avery, Callensen (bib16) 1993; 28
Thompson (bib46) 1997; 9
Cocero, Díaz de la Espina, Aguilar (bib6) 2002; 66
Brown, Radziewic (bib3) 1998; 49
Naitana, Dattena, Gallus, Loi, Branca, Ledda, Cappai (bib28) 1995; 43
Hasler, Henderson, Hurtgen, Jin, McCauley, Mower, Neely, Shuey, Stokes, Trimmer (bib17) 1995; 43
Ohboshi, Fujihara, Yoshida, Tomogane (bib29) 1997; 48
Cognie, Guerin, Guyader, Poulin, Crozet (bib7) 1991; 35
Darvedil, Gustafsson, Shamsuddin, Larsson, Rodriguez-Martínez (bib9) 1994; 35
Scheffen, Van der Zwalmen, Massip (bib37) 1986; 7
Earl, Rowe, Kelly, De Barro (bib12) 1996
Kaidi, Donnay, Van-Langendonckt, Dessy, Massip (bib19) 1998; 52
Zhu, Kasai, Otoge, Sakurai, Machida (bib51) 1993; 98
Wright, Ellington (bib49) 1995; 44
Massip, Mermillod, Dinnyes (bib27) 1995; 10
Crozet, Huneau, Desmedt, Theron, Szollosi, Torres, Sevellec (bib8) 1987; 16
Kasai, Komi, Takakamo, Tsudera, Sakurai, Machida (bib20) 1990; 89
Kuwayama, Hamano, Nagai (bib21) 1992; 96
Prather, Spire, Schalles (bib34) 1987; 28
Leon, Bogliolo, Berlinguer, Rosati, Pintus, Ledda, Naitana (bib24) 2002; 45
Ptak, Dattena, Loi, Tischner, Cappai (bib35) 1999; 15
Leibo, Loskutoff (bib23) 1993; 39
Palasz, Mapletoft (bib30) 1996; 14
Sommerfeld, Niemann (bib38) 1999; 38
Dattena, Ptak, Loi, Cappai (bib10) 2000; 53
Baldassarre, Furnus, de Matos, Pessi (bib2) 1996; 45
Dinnyes, Lonergan, Fair, Boland, Yang (bib11) 1999; 53
Massip, Van der Zwalmen, Scheffen, Ectors (bib26) 1986; 7
Tachikawa, Otoi, Kondo, Machida, Kasai (bib42) 1993; 34
Fahy, Mac Farlane, Angell, Meryman (bib13) 1984; 21
Songsasen, Walmsley, Pollard, Martino, Buckrell, Leibo (bib40) 1996; 76
Gardner, Lane, Spitzer, Batt (bib15) 1994; 50
Catt, O’Brien, Maxwell, Evans (bib4) 1997; 48
Farin, Farin (bib14) 1995; 52
Sommerfeld (10.1016/j.smallrumres.2005.03.002_bib38) 1999; 38
Massip (10.1016/j.smallrumres.2005.03.002_bib26) 1986; 7
Cocero (10.1016/j.smallrumres.2005.03.002_bib6) 2002; 66
Earl (10.1016/j.smallrumres.2005.03.002_bib12) 1996
Darvedil (10.1016/j.smallrumres.2005.03.002_bib9) 1994; 35
Dattena (10.1016/j.smallrumres.2005.03.002_bib10) 2000; 53
Yang (10.1016/j.smallrumres.2005.03.002_bib50) 1992; 37
Fahy (10.1016/j.smallrumres.2005.03.002_bib13) 1984; 21
Leon (10.1016/j.smallrumres.2005.03.002_bib24) 2002; 45
Dinnyes (10.1016/j.smallrumres.2005.03.002_bib11) 1999; 53
Gardner (10.1016/j.smallrumres.2005.03.002_bib15) 1994; 50
Kuwayama (10.1016/j.smallrumres.2005.03.002_bib21) 1992; 96
Ohboshi (10.1016/j.smallrumres.2005.03.002_bib29) 1997; 48
Tachikawa (10.1016/j.smallrumres.2005.03.002_bib42) 1993; 34
Farin (10.1016/j.smallrumres.2005.03.002_bib14) 1995; 52
Songsasen (10.1016/j.smallrumres.2005.03.002_bib39) 1995; 32
Baldassarre (10.1016/j.smallrumres.2005.03.002_bib2) 1996; 45
Tervit (10.1016/j.smallrumres.2005.03.002_bib43) 1984; 21
Winterberger-Torres (10.1016/j.smallrumres.2005.03.002_bib48) 1987
Holm (10.1016/j.smallrumres.2005.03.002_bib18) 1996; 107
Greve (10.1016/j.smallrumres.2005.03.002_bib16) 1993; 28
Ledda (10.1016/j.smallrumres.2005.03.002_bib22) 1999; 77
Catt (10.1016/j.smallrumres.2005.03.002_bib4) 1997; 48
Leibo (10.1016/j.smallrumres.2005.03.002_bib23) 1993; 39
Palasz (10.1016/j.smallrumres.2005.03.002_bib30) 1996; 14
Széll (10.1016/j.smallrumres.2005.03.002_bib41) 1989; 26
Kasai (10.1016/j.smallrumres.2005.03.002_bib20) 1990; 89
Scheffen (10.1016/j.smallrumres.2005.03.002_bib37) 1986; 7
Wright (10.1016/j.smallrumres.2005.03.002_bib49) 1995; 44
Parrish (10.1016/j.smallrumres.2005.03.002_bib32) 1986; 25
Mahmoudzadeh (10.1016/j.smallrumres.2005.03.002_bib25) 1995; 103
Thompson (10.1016/j.smallrumres.2005.03.002_bib45) 1995; 53
Walker (10.1016/j.smallrumres.2005.03.002_bib47) 1996; 45
Cognie (10.1016/j.smallrumres.2005.03.002_bib7) 1991; 35
Kaidi (10.1016/j.smallrumres.2005.03.002_bib19) 1998; 52
Crozet (10.1016/j.smallrumres.2005.03.002_bib8) 1987; 16
Hasler (10.1016/j.smallrumres.2005.03.002_bib17) 1995; 43
Pollard (10.1016/j.smallrumres.2005.03.002_bib33) 1994; 41
Baldassarre (10.1016/j.smallrumres.2005.03.002_bib1) 1992
Rall (10.1016/j.smallrumres.2005.03.002_bib36) 1987; 24
Zhu (10.1016/j.smallrumres.2005.03.002_bib51) 1993; 98
Papadopoulos (10.1016/j.smallrumres.2005.03.002_bib31) 2002; 74
Ptak (10.1016/j.smallrumres.2005.03.002_bib35) 1999; 15
Tervit (10.1016/j.smallrumres.2005.03.002_bib44) 1972; 30
Massip (10.1016/j.smallrumres.2005.03.002_bib27) 1995; 10
Cheng (10.1016/j.smallrumres.2005.03.002_bib5) 1986; 25
Brown (10.1016/j.smallrumres.2005.03.002_bib3) 1998; 49
Naitana (10.1016/j.smallrumres.2005.03.002_bib28) 1995; 43
Prather (10.1016/j.smallrumres.2005.03.002_bib34) 1987; 28
Thompson (10.1016/j.smallrumres.2005.03.002_bib46) 1997; 9
Songsasen (10.1016/j.smallrumres.2005.03.002_bib40) 1996; 76
References_xml – volume: 25
  start-page: 146
  year: 1986
  end-page: 149
  ident: bib5
  article-title: In vitro fertilization of pig and sheep oocytes matured in vivo and in vitro
  publication-title: Theriogenology
  contributor:
    fullname: Polge
– volume: 76
  start-page: 465
  year: 1996
  end-page: 467
  ident: bib40
  article-title: Lambs produced from cryopreserved sheep embryos derived by in vitro fertilization of aspirated oocytes
  publication-title: Can. J. Anim. Sci.
  contributor:
    fullname: Leibo
– volume: 39
  start-page: 81
  year: 1993
  end-page: 94
  ident: bib23
  article-title: Cryobiology of in-vitro-derived bovine embryos
  publication-title: Theriogenology
  contributor:
    fullname: Loskutoff
– volume: 45
  start-page: 111
  year: 1996
  end-page: 120
  ident: bib47
  article-title: The production of unusually large offspring following embryo manipulation: concepts and challenges
  publication-title: Theriogenology
  contributor:
    fullname: Seamark
– volume: 98
  start-page: 139
  year: 1993
  end-page: 145
  ident: bib51
  article-title: Cryopreserved of expanded mouse blastocysts by vitrification in ethylene glycol-based solution
  publication-title: J. Reprod. Fertil.
  contributor:
    fullname: Machida
– volume: 45
  start-page: 204
  year: 2002
  end-page: 212
  ident: bib24
  article-title: Defined media for vitrification, warming, and rehydration: effects on post-thaw protein synthesis and viability of in vitro derived ovine embryos
  publication-title: Cryobiology
  contributor:
    fullname: Naitana
– volume: 9
  start-page: 341
  year: 1997
  end-page: 354
  ident: bib46
  article-title: Comparison between in vivo derived and in vitro-produced pre-elongation embryos from domestic ruminants (review)
  publication-title: Reprod. Fertil. Dev.
  contributor:
    fullname: Thompson
– volume: 77
  start-page: 2234
  year: 1999
  end-page: 2239
  ident: bib22
  article-title: Production and lambing rate of blastocysts derived from in vitro matured oocytes after gonadotropin treatment of prepubertal ewes
  publication-title: J. Anim. Sci.
  contributor:
    fullname: Naitana
– volume: 48
  start-page: 27
  year: 1997
  end-page: 36
  ident: bib29
  article-title: Usefulness of polyethylene glycol for cryopreservation by vitrification of in vitro derived bovine blastocysts
  publication-title: Anim. Reprod. Sci.
  contributor:
    fullname: Tomogane
– year: 1987
  ident: bib48
  article-title: Atlas of the Early Development of the Sheep Embryo
  contributor:
    fullname: Sevellec
– volume: 32
  start-page: 78
  year: 1995
  end-page: 91
  ident: bib39
  article-title: In vitro and in vivo survival of cryopreserved sheep embryos
  publication-title: Cryobiology
  contributor:
    fullname: Leibo
– volume: 24
  start-page: 387
  year: 1987
  end-page: 402
  ident: bib36
  article-title: Factors affecting the survival of mouse embryos cryopreserved by vitrification
  publication-title: Cryobiology
  contributor:
    fullname: Rall
– volume: 53
  start-page: 1385
  year: 1995
  end-page: 1391
  ident: bib45
  article-title: Lamb birth weight is affected by culture system utilized during in vitro pre-elongation development of ovine embryos
  publication-title: Biol. Reprod.
  contributor:
    fullname: Tervit
– volume: 50
  start-page: 390
  year: 1994
  end-page: 400
  ident: bib15
  article-title: Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: amino acids, vitamins, and culturing embryos in groups stimulated development
  publication-title: Biol. Reprod.
  contributor:
    fullname: Batt
– volume: 30
  start-page: 493
  year: 1972
  end-page: 497
  ident: bib44
  article-title: Successful culture in vitro of sheep and cattle ova
  publication-title: J. Reprod. Fertil.
  contributor:
    fullname: Rowson
– volume: 43
  start-page: 141
  year: 1995
  end-page: 152
  ident: bib17
  article-title: Production freezing and transfer of bovine IVF embryos and subsequent calving results
  publication-title: Theriogenology
  contributor:
    fullname: Trimmer
– volume: 21
  start-page: 268
  year: 1984
  ident: bib43
  article-title: Deep-freezing of sheep embryos
  publication-title: Theriogenology
  contributor:
    fullname: Gold
– volume: 45
  start-page: 707
  year: 1996
  end-page: 717
  ident: bib2
  article-title: In vitro production of sheep embryos using laparoscopic folliculocentesis: alternative gonadotrophin treatments for stimulation of oocyte donors
  publication-title: Theriogenology
  contributor:
    fullname: Pessi
– volume: 28
  start-page: 195
  year: 1987
  end-page: 204
  ident: bib34
  article-title: Evaluation of cryopreservation techniques for bovine embryos
  publication-title: Theriogenology
  contributor:
    fullname: Schalles
– volume: 53
  start-page: 318
  year: 1999
  end-page: 324
  ident: bib11
  article-title: Timing of the first cleavage post-insemination affects cryosurvival of in vitro-produced bovine blastocysts
  publication-title: Mol. Reprod. Dev.
  contributor:
    fullname: Yang
– volume: 52
  start-page: 676
  year: 1995
  end-page: 682
  ident: bib14
  article-title: Transfer of bovine embryos produced in vivo or in vitro: survival and fetal development
  publication-title: Biol. Reprod.
  contributor:
    fullname: Farin
– volume: 14
  start-page: 127
  year: 1996
  end-page: 149
  ident: bib30
  article-title: Cryopreservation of mammalian embryos and oocytes: recent advances
  publication-title: Biotech. Adv.
  contributor:
    fullname: Mapletoft
– volume: 21
  start-page: 407
  year: 1984
  end-page: 426
  ident: bib13
  article-title: Vitrification as an approach to cryopreservation
  publication-title: Cryobiology
  contributor:
    fullname: Meryman
– volume: 34
  start-page: 266
  year: 1993
  end-page: 271
  ident: bib42
  article-title: Successful vitrification of bovine blastocysts, derived by in-vitro maturation and fertilization
  publication-title: Mol. Reprod. Dev.
  contributor:
    fullname: Kasai
– volume: 10
  start-page: 3004
  year: 1995
  end-page: 3011
  ident: bib27
  article-title: Morphology and biochemistry if in-vitro-produced bovine embryos: implications for their cryopreservation
  publication-title: Hum. Reprod.
  contributor:
    fullname: Dinnyes
– volume: 28
  start-page: 164
  year: 1993
  end-page: 169
  ident: bib16
  article-title: Viability of in-vivo and on vitro-produced bovine embryos
  publication-title: Reprod. Dom. Anim.
  contributor:
    fullname: Callensen
– volume: 96
  start-page: 187
  year: 1992
  end-page: 193
  ident: bib21
  article-title: Vitrification of bovine blastocysts obtained by in vitro culture of oocytes matured in-vitro
  publication-title: J. Reprod. Fertil.
  contributor:
    fullname: Nagai
– volume: 43
  start-page: 1371
  year: 1995
  end-page: 1378
  ident: bib28
  article-title: Recipient synchronization affects viability of vitrified ovine blastocysts
  publication-title: Theriogenology
  contributor:
    fullname: Cappai
– volume: 26
  start-page: 297
  year: 1989
  end-page: 301
  ident: bib41
  article-title: Osmotic characteristics of sheep and cattle embryos
  publication-title: Cryobiology
  contributor:
    fullname: Széll
– volume: 7
  start-page: 260
  year: 1986
  end-page: 269
  ident: bib37
  article-title: A simple and efficient procedure for preservation of mouse embryos by vitrification
  publication-title: Cryo Letters
  contributor:
    fullname: Massip
– volume: 103
  start-page: 33
  year: 1995
  end-page: 39
  ident: bib25
  article-title: Optimization of a simple vitrification procedure for bovine embryos produced in-vitro: effect of developmental stage, two-step addition of cryoprotectant and sucrose dilution on embryonic survival
  publication-title: J. Reprod. Fertil.
  contributor:
    fullname: de Kruif
– volume: 66
  start-page: 1244
  year: 2002
  end-page: 1258
  ident: bib6
  article-title: Ultraestructural characteristics of fresh an frozen–thawed ovine embryos using two cryoprotectants
  publication-title: Biol. Reprod.
  contributor:
    fullname: Aguilar
– volume: 89
  start-page: 91
  year: 1990
  end-page: 97
  ident: bib20
  article-title: A simple method, for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability
  publication-title: J. Reprod. Fertil.
  contributor:
    fullname: Machida
– volume: 15
  start-page: 1105
  year: 1999
  end-page: 1114
  ident: bib35
  article-title: Ovum pick-up in sheep: efficiency of in vitro embryo production vitrification and birth of offspring
  publication-title: Theriogenology
  contributor:
    fullname: Cappai
– volume: 16
  start-page: 159
  year: 1987
  end-page: 170
  ident: bib8
  article-title: In vitro fertilization with normal development in the sheep
  publication-title: Gamete Res.
  contributor:
    fullname: Sevellec
– volume: 25
  start-page: 591
  year: 1986
  end-page: 600
  ident: bib32
  article-title: Bovine in vitro fertilization with frozen thawed semen
  publication-title: Theriogenology
  contributor:
    fullname: First
– volume: 41
  start-page: 101
  year: 1994
  end-page: 106
  ident: bib33
  article-title: Chilling sensitivity of mammalian embryos
  publication-title: Theriogenology
  contributor:
    fullname: Leibo
– volume: 35
  start-page: 417
  year: 1994
  end-page: 426
  ident: bib9
  article-title: Survival rate and ultrastructure of vitrified bovine in vitro and in vivo developed embryos
  publication-title: Acta Vet. Scan.
  contributor:
    fullname: Rodriguez-Martínez
– volume: 52
  start-page: 39
  year: 1998
  end-page: 50
  ident: bib19
  article-title: Comparison of two co-culture systems to assess the survival of in vitro-produced bovine blastocysts after vitrification
  publication-title: Anim. Reprod. Sci.
  contributor:
    fullname: Massip
– start-page: 182
  year: 1992
  end-page: 183
  ident: bib1
  article-title: Comparative study between human menopausic gonadotrophin (HMG) and hipofisiary follicle stimulating hormone (FSH-P) for superovulation in sheep
  publication-title: Proceedings of the 12th International Congress on Animal Reproduction, vol. 1
  contributor:
    fullname: de Matos
– volume: 44
  start-page: 1167
  year: 1995
  end-page: 1189
  ident: bib49
  article-title: Morphological and physiological differences between in vivo and in vitro-produced preimplantation embryos from livestock species
  publication-title: Theriogenology
  contributor:
    fullname: Ellington
– volume: 49
  start-page: 1525
  year: 1998
  end-page: 1536
  ident: bib3
  article-title: Production of sheep embryos in vitro and development of progeny following single and twin embryo transfers
  publication-title: Theriogenology
  contributor:
    fullname: Radziewic
– volume: 48
  start-page: 1369
  year: 1997
  end-page: 1378
  ident: bib4
  article-title: Effects of rate of development of in vitro-produced ovine embryos on sex ratio and in vitro survival after embryo transfer
  publication-title: Theriogenology
  contributor:
    fullname: Evans
– start-page: 17
  year: 1996
  end-page: 18
  ident: bib12
  article-title: Pregnacy rates for fresh and frozen ovine IVP embryos from juvenile and adult donors
  publication-title: Proceedings of the 13th International Congress on Animal Reproduction
  contributor:
    fullname: De Barro
– volume: 7
  start-page: 270
  year: 1986
  end-page: 273
  ident: bib26
  article-title: Pregnacies following transfer of cattle embryos preserved by vitrification
  publication-title: Cryo Letters
  contributor:
    fullname: Ectors
– volume: 35
  start-page: 393
  year: 1991
  end-page: 400
  ident: bib7
  article-title: In vitro fertilization of sheep oocytes matured in vivo
  publication-title: Theriogenology
  contributor:
    fullname: Crozet
– volume: 74
  start-page: 35
  year: 2002
  end-page: 44
  ident: bib31
  article-title: Embryo survival and recipient pregnancy rates after transfer of fresh or vitrified, in vivo or in vitro-produced ovine blastocysts
  publication-title: Anim. Reprod. Sci.
  contributor:
    fullname: Lonergan
– volume: 107
  start-page: 175
  year: 1996
  end-page: 181
  ident: bib18
  article-title: Embryo viability duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo
  publication-title: J. Reprod. Fertil.
  contributor:
    fullname: Seamark
– volume: 53
  start-page: 1511
  year: 2000
  end-page: 1519
  ident: bib10
  article-title: Survival and viability of vitrified in vitro and in vivo-produced blastocysts
  publication-title: Theriogenology
  contributor:
    fullname: Cappai
– volume: 38
  start-page: 95
  year: 1999
  end-page: 105
  ident: bib38
  article-title: Cryopreservation of bovine in vitro-produced embryos using ethylene glycol in controlled freezing or vitrification
  publication-title: Cryobiology
  contributor:
    fullname: Niemann
– volume: 37
  start-page: 326
  year: 1992
  ident: bib50
  article-title: Vitrification of bovine blastocysts produced in-vitro
  publication-title: Theriogenology
  contributor:
    fullname: Polge
– volume: 21
  start-page: 268
  year: 1984
  ident: 10.1016/j.smallrumres.2005.03.002_bib43
  article-title: Deep-freezing of sheep embryos
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(84)90368-6
  contributor:
    fullname: Tervit
– volume: 26
  start-page: 297
  year: 1989
  ident: 10.1016/j.smallrumres.2005.03.002_bib41
  article-title: Osmotic characteristics of sheep and cattle embryos
  publication-title: Cryobiology
  doi: 10.1016/0011-2240(89)90026-6
  contributor:
    fullname: Széll
– volume: 52
  start-page: 676
  year: 1995
  ident: 10.1016/j.smallrumres.2005.03.002_bib14
  article-title: Transfer of bovine embryos produced in vivo or in vitro: survival and fetal development
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod52.3.676
  contributor:
    fullname: Farin
– volume: 43
  start-page: 141
  year: 1995
  ident: 10.1016/j.smallrumres.2005.03.002_bib17
  article-title: Production freezing and transfer of bovine IVF embryos and subsequent calving results
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(94)00020-U
  contributor:
    fullname: Hasler
– volume: 16
  start-page: 159
  year: 1987
  ident: 10.1016/j.smallrumres.2005.03.002_bib8
  article-title: In vitro fertilization with normal development in the sheep
  publication-title: Gamete Res.
  doi: 10.1002/mrd.1120160207
  contributor:
    fullname: Crozet
– volume: 10
  start-page: 3004
  year: 1995
  ident: 10.1016/j.smallrumres.2005.03.002_bib27
  article-title: Morphology and biochemistry if in-vitro-produced bovine embryos: implications for their cryopreservation
  publication-title: Hum. Reprod.
  doi: 10.1093/oxfordjournals.humrep.a135837
  contributor:
    fullname: Massip
– volume: 21
  start-page: 407
  year: 1984
  ident: 10.1016/j.smallrumres.2005.03.002_bib13
  article-title: Vitrification as an approach to cryopreservation
  publication-title: Cryobiology
  doi: 10.1016/0011-2240(84)90079-8
  contributor:
    fullname: Fahy
– volume: 28
  start-page: 164
  year: 1993
  ident: 10.1016/j.smallrumres.2005.03.002_bib16
  article-title: Viability of in-vivo and on vitro-produced bovine embryos
  publication-title: Reprod. Dom. Anim.
  doi: 10.1111/j.1439-0531.1993.tb00113.x
  contributor:
    fullname: Greve
– start-page: 17
  year: 1996
  ident: 10.1016/j.smallrumres.2005.03.002_bib12
  article-title: Pregnacy rates for fresh and frozen ovine IVP embryos from juvenile and adult donors
  contributor:
    fullname: Earl
– volume: 14
  start-page: 127
  year: 1996
  ident: 10.1016/j.smallrumres.2005.03.002_bib30
  article-title: Cryopreservation of mammalian embryos and oocytes: recent advances
  publication-title: Biotech. Adv.
  doi: 10.1016/0734-9750(96)00005-5
  contributor:
    fullname: Palasz
– volume: 45
  start-page: 111
  year: 1996
  ident: 10.1016/j.smallrumres.2005.03.002_bib47
  article-title: The production of unusually large offspring following embryo manipulation: concepts and challenges
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(95)00360-K
  contributor:
    fullname: Walker
– volume: 7
  start-page: 260
  year: 1986
  ident: 10.1016/j.smallrumres.2005.03.002_bib37
  article-title: A simple and efficient procedure for preservation of mouse embryos by vitrification
  publication-title: Cryo Letters
  contributor:
    fullname: Scheffen
– volume: 107
  start-page: 175
  year: 1996
  ident: 10.1016/j.smallrumres.2005.03.002_bib18
  article-title: Embryo viability duration of gestation and birth weight in sheep after transfer of in vitro matured and in vitro fertilized zygotes cultured in vitro or in vivo
  publication-title: J. Reprod. Fertil.
  doi: 10.1530/jrf.0.1070175
  contributor:
    fullname: Holm
– volume: 25
  start-page: 146
  year: 1986
  ident: 10.1016/j.smallrumres.2005.03.002_bib5
  article-title: In vitro fertilization of pig and sheep oocytes matured in vivo and in vitro
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(86)90200-1
  contributor:
    fullname: Cheng
– volume: 39
  start-page: 81
  year: 1993
  ident: 10.1016/j.smallrumres.2005.03.002_bib23
  article-title: Cryobiology of in-vitro-derived bovine embryos
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(93)90025-Z
  contributor:
    fullname: Leibo
– volume: 48
  start-page: 1369
  year: 1997
  ident: 10.1016/j.smallrumres.2005.03.002_bib4
  article-title: Effects of rate of development of in vitro-produced ovine embryos on sex ratio and in vitro survival after embryo transfer
  publication-title: Theriogenology
  doi: 10.1016/S0093-691X(97)00378-6
  contributor:
    fullname: Catt
– volume: 52
  start-page: 39
  year: 1998
  ident: 10.1016/j.smallrumres.2005.03.002_bib19
  article-title: Comparison of two co-culture systems to assess the survival of in vitro-produced bovine blastocysts after vitrification
  publication-title: Anim. Reprod. Sci.
  doi: 10.1016/S0378-4320(98)00089-X
  contributor:
    fullname: Kaidi
– volume: 103
  start-page: 33
  year: 1995
  ident: 10.1016/j.smallrumres.2005.03.002_bib25
  article-title: Optimization of a simple vitrification procedure for bovine embryos produced in-vitro: effect of developmental stage, two-step addition of cryoprotectant and sucrose dilution on embryonic survival
  publication-title: J. Reprod. Fertil.
  doi: 10.1530/jrf.0.1030033
  contributor:
    fullname: Mahmoudzadeh
– volume: 30
  start-page: 493
  year: 1972
  ident: 10.1016/j.smallrumres.2005.03.002_bib44
  article-title: Successful culture in vitro of sheep and cattle ova
  publication-title: J. Reprod. Fertil.
  doi: 10.1530/jrf.0.0300493
  contributor:
    fullname: Tervit
– volume: 53
  start-page: 1385
  year: 1995
  ident: 10.1016/j.smallrumres.2005.03.002_bib45
  article-title: Lamb birth weight is affected by culture system utilized during in vitro pre-elongation development of ovine embryos
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod53.6.1385
  contributor:
    fullname: Thompson
– volume: 37
  start-page: 326
  year: 1992
  ident: 10.1016/j.smallrumres.2005.03.002_bib50
  article-title: Vitrification of bovine blastocysts produced in-vitro
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(92)90395-8
  contributor:
    fullname: Yang
– volume: 45
  start-page: 707
  year: 1996
  ident: 10.1016/j.smallrumres.2005.03.002_bib2
  article-title: In vitro production of sheep embryos using laparoscopic folliculocentesis: alternative gonadotrophin treatments for stimulation of oocyte donors
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(95)00417-7
  contributor:
    fullname: Baldassarre
– start-page: 182
  year: 1992
  ident: 10.1016/j.smallrumres.2005.03.002_bib1
  article-title: Comparative study between human menopausic gonadotrophin (HMG) and hipofisiary follicle stimulating hormone (FSH-P) for superovulation in sheep
  contributor:
    fullname: Baldassarre
– volume: 50
  start-page: 390
  year: 1994
  ident: 10.1016/j.smallrumres.2005.03.002_bib15
  article-title: Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: amino acids, vitamins, and culturing embryos in groups stimulated development
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod50.2.390
  contributor:
    fullname: Gardner
– volume: 7
  start-page: 270
  year: 1986
  ident: 10.1016/j.smallrumres.2005.03.002_bib26
  article-title: Pregnacies following transfer of cattle embryos preserved by vitrification
  publication-title: Cryo Letters
  contributor:
    fullname: Massip
– volume: 41
  start-page: 101
  year: 1994
  ident: 10.1016/j.smallrumres.2005.03.002_bib33
  article-title: Chilling sensitivity of mammalian embryos
  publication-title: Theriogenology
  doi: 10.1016/S0093-691X(05)80054-8
  contributor:
    fullname: Pollard
– volume: 48
  start-page: 27
  year: 1997
  ident: 10.1016/j.smallrumres.2005.03.002_bib29
  article-title: Usefulness of polyethylene glycol for cryopreservation by vitrification of in vitro derived bovine blastocysts
  publication-title: Anim. Reprod. Sci.
  doi: 10.1016/S0378-4320(97)00034-1
  contributor:
    fullname: Ohboshi
– year: 1987
  ident: 10.1016/j.smallrumres.2005.03.002_bib48
  contributor:
    fullname: Winterberger-Torres
– volume: 32
  start-page: 78
  year: 1995
  ident: 10.1016/j.smallrumres.2005.03.002_bib39
  article-title: In vitro and in vivo survival of cryopreserved sheep embryos
  publication-title: Cryobiology
  doi: 10.1006/cryo.1995.1007
  contributor:
    fullname: Songsasen
– volume: 66
  start-page: 1244
  year: 2002
  ident: 10.1016/j.smallrumres.2005.03.002_bib6
  article-title: Ultraestructural characteristics of fresh an frozen–thawed ovine embryos using two cryoprotectants
  publication-title: Biol. Reprod.
  doi: 10.1095/biolreprod66.5.1244
  contributor:
    fullname: Cocero
– volume: 53
  start-page: 318
  year: 1999
  ident: 10.1016/j.smallrumres.2005.03.002_bib11
  article-title: Timing of the first cleavage post-insemination affects cryosurvival of in vitro-produced bovine blastocysts
  publication-title: Mol. Reprod. Dev.
  doi: 10.1002/(SICI)1098-2795(199907)53:3<318::AID-MRD7>3.0.CO;2-O
  contributor:
    fullname: Dinnyes
– volume: 74
  start-page: 35
  year: 2002
  ident: 10.1016/j.smallrumres.2005.03.002_bib31
  article-title: Embryo survival and recipient pregnancy rates after transfer of fresh or vitrified, in vivo or in vitro-produced ovine blastocysts
  publication-title: Anim. Reprod. Sci.
  doi: 10.1016/S0378-4320(02)00162-8
  contributor:
    fullname: Papadopoulos
– volume: 38
  start-page: 95
  year: 1999
  ident: 10.1016/j.smallrumres.2005.03.002_bib38
  article-title: Cryopreservation of bovine in vitro-produced embryos using ethylene glycol in controlled freezing or vitrification
  publication-title: Cryobiology
  doi: 10.1006/cryo.1999.2159
  contributor:
    fullname: Sommerfeld
– volume: 77
  start-page: 2234
  year: 1999
  ident: 10.1016/j.smallrumres.2005.03.002_bib22
  article-title: Production and lambing rate of blastocysts derived from in vitro matured oocytes after gonadotropin treatment of prepubertal ewes
  publication-title: J. Anim. Sci.
  doi: 10.2527/1999.7782234x
  contributor:
    fullname: Ledda
– volume: 35
  start-page: 393
  year: 1991
  ident: 10.1016/j.smallrumres.2005.03.002_bib7
  article-title: In vitro fertilization of sheep oocytes matured in vivo
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(91)90289-P
  contributor:
    fullname: Cognie
– volume: 49
  start-page: 1525
  year: 1998
  ident: 10.1016/j.smallrumres.2005.03.002_bib3
  article-title: Production of sheep embryos in vitro and development of progeny following single and twin embryo transfers
  publication-title: Theriogenology
  doi: 10.1016/S0093-691X(98)00098-3
  contributor:
    fullname: Brown
– volume: 98
  start-page: 139
  year: 1993
  ident: 10.1016/j.smallrumres.2005.03.002_bib51
  article-title: Cryopreserved of expanded mouse blastocysts by vitrification in ethylene glycol-based solution
  publication-title: J. Reprod. Fertil.
  doi: 10.1530/jrf.0.0980139
  contributor:
    fullname: Zhu
– volume: 96
  start-page: 187
  year: 1992
  ident: 10.1016/j.smallrumres.2005.03.002_bib21
  article-title: Vitrification of bovine blastocysts obtained by in vitro culture of oocytes matured in-vitro
  publication-title: J. Reprod. Fertil.
  doi: 10.1530/jrf.0.0960187
  contributor:
    fullname: Kuwayama
– volume: 9
  start-page: 341
  year: 1997
  ident: 10.1016/j.smallrumres.2005.03.002_bib46
  article-title: Comparison between in vivo derived and in vitro-produced pre-elongation embryos from domestic ruminants (review)
  publication-title: Reprod. Fertil. Dev.
  doi: 10.1071/R96079
  contributor:
    fullname: Thompson
– volume: 24
  start-page: 387
  year: 1987
  ident: 10.1016/j.smallrumres.2005.03.002_bib36
  article-title: Factors affecting the survival of mouse embryos cryopreserved by vitrification
  publication-title: Cryobiology
  doi: 10.1016/0011-2240(87)90042-3
  contributor:
    fullname: Rall
– volume: 45
  start-page: 204
  year: 2002
  ident: 10.1016/j.smallrumres.2005.03.002_bib24
  article-title: Defined media for vitrification, warming, and rehydration: effects on post-thaw protein synthesis and viability of in vitro derived ovine embryos
  publication-title: Cryobiology
  doi: 10.1016/S0011-2240(02)00139-6
  contributor:
    fullname: Leon
– volume: 53
  start-page: 1511
  year: 2000
  ident: 10.1016/j.smallrumres.2005.03.002_bib10
  article-title: Survival and viability of vitrified in vitro and in vivo-produced blastocysts
  publication-title: Theriogenology
  doi: 10.1016/S0093-691X(00)00293-4
  contributor:
    fullname: Dattena
– volume: 35
  start-page: 417
  year: 1994
  ident: 10.1016/j.smallrumres.2005.03.002_bib9
  article-title: Survival rate and ultrastructure of vitrified bovine in vitro and in vivo developed embryos
  publication-title: Acta Vet. Scan.
  doi: 10.1186/BF03548317
  contributor:
    fullname: Darvedil
– volume: 15
  start-page: 1105
  year: 1999
  ident: 10.1016/j.smallrumres.2005.03.002_bib35
  article-title: Ovum pick-up in sheep: efficiency of in vitro embryo production vitrification and birth of offspring
  publication-title: Theriogenology
  doi: 10.1016/S0093-691X(99)00198-3
  contributor:
    fullname: Ptak
– volume: 76
  start-page: 465
  year: 1996
  ident: 10.1016/j.smallrumres.2005.03.002_bib40
  article-title: Lambs produced from cryopreserved sheep embryos derived by in vitro fertilization of aspirated oocytes
  publication-title: Can. J. Anim. Sci.
  doi: 10.4141/cjas96-069
  contributor:
    fullname: Songsasen
– volume: 43
  start-page: 1371
  year: 1995
  ident: 10.1016/j.smallrumres.2005.03.002_bib28
  article-title: Recipient synchronization affects viability of vitrified ovine blastocysts
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(95)00122-O
  contributor:
    fullname: Naitana
– volume: 89
  start-page: 91
  year: 1990
  ident: 10.1016/j.smallrumres.2005.03.002_bib20
  article-title: A simple method, for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability
  publication-title: J. Reprod. Fertil.
  doi: 10.1530/jrf.0.0890091
  contributor:
    fullname: Kasai
– volume: 44
  start-page: 1167
  year: 1995
  ident: 10.1016/j.smallrumres.2005.03.002_bib49
  article-title: Morphological and physiological differences between in vivo and in vitro-produced preimplantation embryos from livestock species
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(95)00327-5
  contributor:
    fullname: Wright
– volume: 25
  start-page: 591
  year: 1986
  ident: 10.1016/j.smallrumres.2005.03.002_bib32
  article-title: Bovine in vitro fertilization with frozen thawed semen
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(86)90143-3
  contributor:
    fullname: Parrish
– volume: 28
  start-page: 195
  year: 1987
  ident: 10.1016/j.smallrumres.2005.03.002_bib34
  article-title: Evaluation of cryopreservation techniques for bovine embryos
  publication-title: Theriogenology
  doi: 10.1016/0093-691X(87)90266-4
  contributor:
    fullname: Prather
– volume: 34
  start-page: 266
  year: 1993
  ident: 10.1016/j.smallrumres.2005.03.002_bib42
  article-title: Successful vitrification of bovine blastocysts, derived by in-vitro maturation and fertilization
  publication-title: Mol. Reprod. Dev.
  doi: 10.1002/mrd.1080340306
  contributor:
    fullname: Tachikawa
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Snippet The purpose of this study was to evaluate different cryopreservation protocols for in vitro-produced ovine embryos and assess the survival rate after...
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SubjectTerms cell nucleus
cryopreservation
cryoprotectants
culture media
embryo (animal)
embryo hatching rate
embryogenesis
embryonic mortality
ethylene glycol
fetal death
glycerol
in vitro fertilization
In vitro survival
Lambing
lambing rate
sheep
sucrose
Transfer
vitrification
Title Cryopreservation of in vitro-produced ovine embryos
URI https://dx.doi.org/10.1016/j.smallrumres.2005.03.002
Volume 63
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