Determination of nitrate reductase activity in barley leaves and roots

• Published in: Annals of botany Vol. 49; no. 1; pp. 31 - 37
• Main Authors: Lewis, O.A.M, Watson, E.F, Hewitt, E.J
• Format: Journal Article
• Language: English
• Published: Oxford University Press 1982; Academic Press Inc
• Subjects: Barley; Corn; Enzymes; Hordeum vulgare L; Leaves; Memory interference; nitrate reductase; Nitrates; Nitrites; plant biochemistry; Plant nutrition; plant physiology; Plant roots; Plants

The inactivation of nitrate reductase in the leaves and roots of barley (Hordeum vulgare L. cv. Mazurka) during and after extracting was investigated. At 0 °C in the absence of casein, 25 per cent of ‘total’. i.e. maximal in vitro, nitrate reductase activity was lost during the 2 min extraction proc...