Electron Microscopic Visualization of Sites of Nascent DNA Synthesis by Streptavidin-Gold Binding to Biotinylated Nucleotides Incorporated in Vivo

• Published in: The Journal of cell biology Vol. 107; no. 1; pp. 33 - 44
• Main Authors: Hiriyanna, K. T., Varkey, J., Beer, M., Benbow, R. M.
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.07.1988; The Rockefeller University Press
• Subjects: Animals; Autoradiography; Bacterial Proteins; Biological and medical sciences; Biotin; Colloids; Deoxyadenine Nucleotides - metabolism; Deoxycytosine Nucleotides - metabolism; Deoxyuracil Nucleotides - metabolism; DNA; DNA - analysis; DNA - biosynthesis; DNA - ultrastructure; Eggs; Electron microscopy; Electrophoresis, Agar Gel; Embryos; Fundamental and applied biological sciences. Psychology; Gels; Gold; Microinjections; Microscopy, Electron; Molecular and cellular biology; Molecular genetics; Molecular weight; Molecules; Nucleosides; Nucleotides; Plasmids; Streptavidin; Xenopus laevis

Biotinylated nucleotides (bio-11-dCTP, bio-11-dUTP, and bio-7-dATP) were microinjected into unfertilized and fertilized Xenopus laevis eggs. The amounts introduced were comparable to in vivo deoxynucleoside triphosphate pools. At various times after microinjection, DNA was extracted from eggs or emb...