Inhibition of polyphosphoinositide breakdown and c-myc induction accompanying inhibition of human B-cell activation by two monoclonal antibodies against the leucocyte common antigen (CD45)

• Published in: Scandinavian journal of immunology Vol. 31; no. 5; p. 583
• Main Authors: Smeland, E B, Holte, H, Kiil Blomhoff, H, Asheim, H C, Stokke, T, Torjesen, P, Funderud, S
• Format: Journal Article
• Language: English
• Published: England 01.05.1990
• Subjects: Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - pharmacology; Antigens, Differentiation - immunology; B-Lymphocytes - immunology; Calcium - metabolism; Cell Cycle - drug effects; Cell Division - drug effects; Histocompatibility Antigens - immunology; Humans; Immunoglobulin mu-Chains - metabolism; Leukocyte Common Antigens; Lymphocyte Activation - drug effects; Lymphocyte Activation - immunology; Lymphokines - immunology; Phosphatidylinositols - metabolism; Proto-Oncogene Proteins - biosynthesis; Proto-Oncogene Proteins c-myc; RNA - biosynthesis; Tetradecanoylphorbol Acetate - pharmacology

In this report we show that the two monoclonal anti-CD45 antibodies, EO-1 and FN-126, potently inhibit G0 to G1 transition and S phase entry in human B cells stimulated with anti-mu and low molecular weight B-cell growth factor. Both antibodies were found to inhibit anti-mu-induced inositol phosphol...