Combining directed evolution with high cell permeability for high-level cadaverine production in engineered Escherichia coli

• Published in: Biotechnology journal Vol. 19; no. 3; p. e2300642
• Main Authors: Liu, Xuemei, Luo, Ruoshi, Wang, Dan, Xiao, Kaixing, Lin, Fanzhen, Kang, Ya Qi, Xia, Xue, Zhou, Xiaojie, Hu, Ge
• Format: Journal Article
• Language: English
• Published: Germany 01.03.2024
• Subjects: Biotransformation; Cadaverine - metabolism; Catalysis; Escherichia coli - genetics; Lipopolysaccharides - metabolism; Lysine - metabolism; cell permeability; lipopolysaccharides; whole-cell catalysis; cadaverine

The biosynthesis of cadaverine from lysine is an environmentally promising technology, that could contribute to a more sustainable approach to manufacturing bio-nylon 5X. However, the titer of biosynthesized cadaverine has still not reached a sufficient level for industrial production. A powerful gr...