Transformation of soybean Gy3 gene into Artemisaarenaria mediated by corona discharge

In order to improve the protein content of desert plant, a method of genetic transformation mediated by corona discharge was established. Artemisia seeds were processed in corona electric field for 120 min at 12 kV, and then soaked in 0.1SSC media that contained Soybean Gy3 gene DNA to incubate for...

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Published inJournal of physics. Conference series Vol. 418; no. 1; pp. 1 - 5
Main Authors Chao, Lu-meng, Na, Ri, Xue, Dan, Xu, Yongze, Liu, Teng
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.01.2013
Subjects
Artemisia
Coronas
Deoxyribonucleic acid
DNA
Electric corona
Electric fields
Genes
Genetic modification
Physics
Polymerase chain reaction
Power plants
Seeds
Soybeans
Transformations
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Summary:In order to improve the protein content of desert plant, a method of genetic transformation mediated by corona discharge was established. Artemisia seeds were processed in corona electric field for 120 min at 12 kV, and then soaked in 0.1SSC media that contained Soybean Gy3 gene DNA to incubate for 12 h at 26 [degrees]C. Finally the seeds were inoculated on the differentiation medium. Polymerase Chain Reaction (PCR) and Reverse Transcription Polymerase Chain Reaction (RT-PCR) detection showed that the Soybean Gy3 gene had been successfully introduced into genomic DNA of the regenerated plants of Artemisaarenaria. The study provided a new way for corona discharge in plant genetic modification.
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ISSN:1742-6588
1742-6596
DOI:10.1088/1742-6596/418/1/012138