Recommendations for the Development of Cell-Based Anti-Viral Vector Neutralizing Antibody Assays

• Published in: The AAPS journal Vol. 22; no. 2; p. 24
• Main Authors: Gorovits, Boris, Fiscella, Michele, Havert, Mike, Koren, Eugen, Long, Brian, Milton, Mark, Purushothama, Shobha
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 06.01.2020
• Subjects: Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Biochemistry; Biological Assay; Biomedical and Life Sciences; Biomedicine; Biotechnology; Cell Line; Dependovirus - genetics; Dependovirus - immunology; Genes, Reporter; Genetic Therapy; Genetic Vectors - genetics; Genetic Vectors - immunology; Humans; Immunologic Tests; Pharmacology/Toxicology; Pharmacy; Predictive Value of Tests; Reproducibility of Results; Review Article; Viral vector Gene Therapy; Anti-Capsid Immune Response; Cell-Based Assay; Neutralizing Antibody

Viral vector–based gene therapies (GTx) have received significant attention in the recent years and the number of ongoing GTx clinical trials is increasing. A platform of choice for many of these studies is adeno-associated virus (AAV). All humans may be exposed to natural AAV infections and could m...