Strategy to mitigate aggregation during Protein A chromatography and low pH virus inactivation for a nivolumab biosimilar candidate - Pargas-Ferrer, Elizabeth; Chang, Sum Lai Lozada; García, Katia; Azaharez, Ernesto; Palacio, Julio; Mena, Midalys Cabrera; Boggiano-Ayo, Tammy (Journal of Chromatography A, 2025)
Keywords: affinity chromatography; Animals; Antibodies, Monoclonal - chemistry; Antibodies, Monoclonal - isolation & purification; arginine; bioactive properties; Chromatography, Affinity - methods; Glycine - chemistry; histidine; Hydrogen-Ion Concentration; immunoglobulin G; mannitol; molecular weight; Monoclonal antibody; Nivolumab; Nivolumab - chemistry; polysorbates; Protein A affinity chromatography; Protein Aggregates; Protein aggregation; Protein purification; sodium citrate; sorbitol; Staphylococcal Protein A - chemistry; sucrose; trehalose; urea; Virus Inactivation; viruses; Protein aggregation; Monoclonal antibody; Protein purification; Nivolumab; Protein A affinity chromatography