Characterization of a Novel Pectate Lyase from Erwinia carotovora subsp. carotovora

• Published in: Molecular plant-microbe interactions Vol. 8; no. 2; pp. 207 - 217
• Main Author: Heikinheimo, Riikka
• Format: Journal Article
• Language: English
• Published: St Paul, MN APS Press 01.03.1995
• Subjects: Amino Acid Sequence; amino acid sequences; Bacterial plant pathogens; Bacteriology; Base Sequence; Biological and medical sciences; chromosome mapping; Cloning, Molecular; DNA, Bacterial; enzyme activity; enzymology; Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Bacterial; Genetics; Isoelectric Focusing; Isoenzymes; Isoenzymes - genetics; Isoenzymes - metabolism; isozymes; metabolism; Microbiology; Molecular Sequence Data; nucleotide sequences; pectate lyase; pectins; Pectobacterium carotovorum; Pectobacterium carotovorum - enzymology; Pectobacterium carotovorum subsp. carotovorum; Phytopathology. Animal pests. Plant and forest protection; polygalacturonase; Polygalacturonase - genetics; Polysaccharide-Lyases; Polysaccharide-Lyases - genetics; Polysaccharide-Lyases - metabolism; structural genes; Systematics. Structure, properties and multiplication. Genetics; transcription (genetics); virulence; Polysaccharide-lyases; Pectate lyase; Plant pathogen; Nucleotide sequence; Enzyme; Isozyme; Gene organization; Lyases; Extracellular; Bacteria; Carbon-oxygen lyases; Aminoacid sequence; Enterobacteriaceae

The pectate lyase (Pel, EC 4.2.2.2) isoenzyme profile of Erwinia carotovora subsp. carotovora was characterized by isoelectric focusing, and the corresponding genes coding for four different exported Pels were cloned. The nucleotide sequence of the pelB gene encoding one of these isoenzymes was dete...