Multiplex Hybrid Capture Improves the Deep Detection of Antimicrobial Resistance Genes from Wastewater Treatment Plant Effluents to Assess Environmental Issues

• Published in: Microbial drug resistance (Larchmont, N.Y.)
• Main Authors: Sekizuka, Tsuyoshi, Yamaguchi, Nobuyasu, Kanamori, Hajime, Kuroda, Makoto
• Format: Journal Article
• Language: English; Japanese
• Published: United States 01.11.2023
• Subjects: antimicrobial resistance gene; hybrid capture; effluent; metagenomics; sewage

Metagenomic sequencing (mDNA-seq) is one of the best approaches to address antimicrobial resistance (AMR) issues and characterize AMR genes (ARGs) and their host bacteria (ARB); however, the sensitivity provided is insufficient for the overall detection in wastewater treatment plant (WWTP) effluents...