Evaluation of stabilizing additives to protect activities of cytochrome P450 enzymes for in vitro drug testing and pharmacogenetic studies: Focus on CYP2D6 - Yamoune, Sabrina; Koch, Henner; Delev, Daniel; Weber, Yvonne; Stingl, Julia Carolin (Biochimica et biophysica acta. General subjects, 2025)
Keywords: Biocatalysis engineering; brain; Brain - enzymology; Brain - metabolism; Brain drug metabolism; CYP in vitro stability; CYP2D6; cysteine; cytochrome P-450; Cytochrome P-450 CYP2D6 - chemistry; Cytochrome P-450 CYP2D6 - metabolism; Cytochrome P-450 Enzyme System - metabolism; dithiothreitol; Dithiothreitol - chemistry; Dithiothreitol - pharmacology; drug development; drugs; Enzyme Stability - drug effects; enzymes; Humans; liver; liver microsomes; Local drug metabolism; Microsomes, Liver - enzymology; Microsomes, Liver - metabolism; organoids; Pharmacogenomic Testing - methods; pharmacokinetics; phosphorylcholine; safety assessment; Brain drug metabolism; DTT; DMSO; SOD; EtOH; Biocatalysis engineering; EDTA; MS/MS; AUC; BSA; CYP in vitro stability; PC; CYP2D6; MeOH; PMSF; Local drug metabolism; ROS; CYP; DLPC; KPB; Cys; GSH; HPLC