Analytical sensitivity and stability of DNA methylation testing in stool samples for colorectal cancer detection

• Published in: Cellular oncology (Dordrecht) Vol. 35; no. 4; pp. 309 - 315
• Main Authors: Bosch, Linda J. W., Mongera, Sandra, sive Droste, Jochim S. Terhaar, Oort, Frank A., van Turenhout, Sietze T., Penning, Maarten T., Louwagie, Joost, Mulder, Chris J. J., van Engeland, Manon, Carvalho, Beatriz, Meijer, Gerrit A.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2012
• Subjects: Base Sequence; beta-Globins - genetics; Biomedical and Life Sciences; Biomedicine; Cancer Research; Cell Cycle Proteins - genetics; Colorectal Neoplasms - diagnosis; Colorectal Neoplasms - genetics; DNA Methylation; DNA Mutational Analysis; DNA, Neoplasm - genetics; Feasibility Studies; Feces - chemistry; Genetic Testing - methods; HCT116 Cells; Humans; Mutation; Neoplasm Proteins - genetics; Oncology; Original Paper; Pathology; Poly-ADP-Ribose Binding Proteins; Polymerase Chain Reaction; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins p21(ras); ras Proteins - genetics; Reproducibility of Results; Sensitivity and Specificity; Temperature; Time Factors; Ubiquitin-Protein Ligases; FIT; Screening; Stool; Analytical sensitivity; Stability; CRC; DNA methylation; iFOBT

Background Stool-based molecular tests hold large potential for improving colorectal cancer screening. Here, we investigated the analytical sensitivity of a DNA methylation assay on partial stool samples, and estimated the DNA degradation in stool over time. In addition, we explored the detection of...