Transcriptomic analysis of HEK293A cells with a CRISPR/Cas9-mediated TDP1 knockout

• Published in: Biochimica et biophysica acta. General subjects Vol. 1868; no. 7; p. 130616
• Main Authors: Dyrkheeva, Nadezhda S., Zakharenko, Alexandra L., Malakhova, Anastasia A., Okorokova, Larisa S., Shtokalo, Dmitry N., Medvedev, Sergey P., Tupikin, Alexey A., Kabilov, Marsel R., Lavrik, Olga I.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2024
• Subjects: cancer therapy; cell adhesion; cell lines; CRISPR-Cas Systems; cytokines; DNA; DNA repair; DNA Repair - genetics; family; Gene Expression Profiling; Gene Knockout Techniques - methods; gene targeting; genes; HEK293 Cells; HEK293A; homozygosity; Humans; mitochondria; neurodegenerative diseases; phospholipases; Phosphoric Diester Hydrolases - genetics; Phosphoric Diester Hydrolases - metabolism; spermatogenesis; TDP1 knockout; Topoisomerase 1; Transcriptome; Transcriptome - genetics; transcriptomics; Tyrosyl-DNA phosphodiesterase 1; Topoisomerase 1; Tyrosyl-DNA phosphodiesterase 1; HEK293A; TDP1 knockout; Transcriptome

Tyrosyl-DNA phosphodiesterase 1 (TDP1) is a human DNA repair protein. It is a member of the phospholipase D family based on structural similarity. TDP1 is a key enzyme of the repair of stalled topoisomerase 1 (TOP1)–DNA complexes. Previously, with the CRISPR/Cas9 method, we obtained HEK293A cells wi...