A novel three-dimensional co-culture model for studying exosome-mediated cell interactions in glioblastoma

• Published in: Biochimica et biophysica acta. General subjects Vol. 1869; no. 3; p. 130752
• Main Authors: Li, Kaishu, Du, Siyuan, Li, Haichao, Li, Zhaohui, Zhu, Qihui, Peng, Qian, Liao, Baojian, Qi, Ling
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2025
• Subjects: Cell Communication; Cell Culture Techniques, Three Dimensional - methods; cell growth; Cell Line, Tumor; Cell Proliferation; centrifugation; Co-culture; coculture; Coculture Techniques - methods; commercialization; drugs; Exosomes; Exosomes - metabolism; fluorescent antibody technique; Glioblastoma; Glioblastoma - metabolism; Glioblastoma - pathology; Humans; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - metabolism; metabolism; microRNA; miRNAs; Three-dimensional cell culture systems; transmission electron microscopy; Tumor Microenvironment; Three-dimensional cell culture systems; miRNAs; Co-culture; Exosomes; Glioblastoma

Three-dimensional(3D) cell culture systems provide a larger space for cell proliferation, which is crucial for simulating cellular behavior and drug responses in the tumor microenvironment. In this study, we developed a novel 3D co-culture system for cell interactions, utilizing a commercialized bio...