In vitro regeneration of adventitious buds from leaf explants and their subsequent cryopreservation in highbush blueberry

• Published in: Plant cell, tissue and organ culture Vol. 134; no. 2; pp. 193 - 204
• Main Authors: Chen, Hai-Yan, Liu, Jing, Pan, Chen, Yu, Jing-Wei, Wang, Qiao-Chun
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2018; Springer Nature B.V
• Subjects: Aluminum; Bearing; Berries; Biomedical and Life Sciences; Blueberries; Buds; Cryopreservation; Cultivars; Dehydration; Deoxyribonucleic acid; DNA; Explants; Foils; Glycerol; Leaves; Life Sciences; Liquid nitrogen; Metal foils; Morphology; Nucleotide sequence; Original Article; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Plantlets; Polymerase chain reaction; Random amplified polymorphic DNA; Regeneration; Regrowth; Shoots; Submerging; Sucrose; Sugar; Tips; Vaccinium corymbosum; Vitrification; Zeatin; Leaf explant; Droplet-vitrification; Recovery; Blueberry; Long-term preservation; Genetic stability

We report a new cryopreservation method for highbush blueberry ( Vaccinium corymbosum ) using small leaf squares-bearing adventitious buds. Leaf explants were cultured on adventitious bud regeneration medium composed of Woody Plant Medium (WPM) supplemented with 20 µM zeatin. After 21 days of advent...