Effects of Mutations in Three Domains of the Vesicular Stomatitis Viral Glycoprotein on Its Lateral Diffusion in the Plasma Membrane

• Published in: The Journal of cell biology Vol. 105; no. 1; pp. 69 - 75
• Main Authors: Scullion, Brian F., Hou, Yu, Puddington, Lynn, Rose, John K., Jacobson, Ken
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.07.1987; The Rockefeller University Press
• Subjects: Amino Acid Sequence; Amino acids; Animals; Biological and medical sciences; Cell coat. Cell surface; Cell Line; Cell membranes; Cell structures and functions; Cells; Cercopithecus aethiops; COS cells; Diffusion; Diffusion coefficient; DNA - genetics; DNA, Viral - genetics; Fibroblasts - metabolism; Fibroblasts - ultrastructure; Fundamental and applied biological sciences. Psychology; Glycoproteins; L cells; Lymphocytes; Membrane Glycoproteins; Membrane proteins; Membrane Proteins - genetics; Membrane Proteins - metabolism; Molecular and cellular biology; Plasma diffusion; Protein Conformation; Protein Processing, Post-Translational; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Vesicular stomatitis Indiana virus - genetics; Vesicular stomatitis Indiana virus - metabolism; Viral Envelope Proteins; Viral Proteins - genetics; Viral Proteins - metabolism; Virus; Cell culture; Vesicular stomatitis virus; Vesiculovirus; Transfection; Rhabdoviridae; Lateral diffusion; Plasma membrane; Site directed mutagenesis; Glycoproteins; Diffusion coefficient

The lateral mobility of the vesicular stomatitis virus spike glycoprotein (G protein) and various mutant G proteins produced by site-directed mutagenesis of the G cDNA has been measured. Fluorescence recovery after photobleaching results for the wild type G protein in transfected COS-1 cells yielded...