Identification of the oxidation states of the active site cysteine in a recombinant protein tyrosine phosphatase by electrospray mass spectrometry using on-line desalting

• Published in: Rapid communications in mass spectrometry Vol. 12; no. 20; pp. 1457 - 1462
• Main Authors: DeGnore, Jon P., König, Simone, Barrett, William C., Chock, P. Boon, Fales, Henry M.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 30.10.1998
• Subjects: Amino Acid Sequence; Binding Sites; Cysteine - chemistry; Humans; Mass Spectrometry; Molecular Sequence Data; Oxidation-Reduction; Protein Tyrosine Phosphatases - chemistry; Recombinant Proteins - analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

The oxidation state of the cysteine residue at the active site of human protein tyrosine phosphatase (PTP‐1B) greatly affects its enzymatic activity. We wished to examine peroxide‐treated preparations for modifications of this enzyme with electrospray mass spectrometry in order to determine the loca...