Immortalization of bovine umbilical vein endothelial cells: a model for the study of vascular endothelium

• Published in: European journal of cell biology Vol. 81; no. 1; pp. 1 - 8
• Main Authors: Cajero-Juárez, Marcos, Avila, Bibiana, Ochoa, Alejandra, Garrido-Guerrero, Efraín, Varela-Echavarría, Alfredo, Martínez de la Escalera, Gonzalo, Clapp, Carmen
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 2002
• Subjects: Animals; Blood Proteins - pharmacology; bovine umbilical vein; Cattle; Cell Culture Techniques - methods; Cell Division - genetics; Cell Line, Transformed - cytology; Cell Line, Transformed - metabolism; Cell Size; Clone Cells - cytology; Clone Cells - metabolism; DNA Probes, HPV - genetics; Dose-Response Relationship, Drug; Endothelium cell line; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Female; Genetic Vectors - genetics; immortalization; Immunohistochemistry; Models, Biological; oncogenes; Papillomaviridae - genetics; Phenotype; Plasmids - genetics; Pregnancy; prolactin; Prolactin - genetics; Prolactin - pharmacology; RNA, Messenger - metabolism; Transfection - methods; Umbilical Veins - cytology; Umbilical Veins - metabolism; Viral Proteins - genetics; Endothelium cell line; oncogenes; bovine umbilical vein; prolactin; immortalization

Endothelial cells perform a large array of physiological functions that are influenced by their cellular heterogeneity in the different vascular beds. Vein endothelial cells isolated from the umbilical cords are commonly used to study vascular endothelium. Primary cultures of these cells, however, h...