Wood cellulignin as an alternative matrix for enzyme immobilization

• Published in: Applied biochemistry and biotechnology Vol. 121; no. 1-3; pp. 255 - 268
• Main Authors: Gomes, F.M, Silva, G.S, Pinati, D.G, Conte, R.A, Castro, H.F. de
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 2005
• Subjects: Biochemistry; Biomass; Candida - enzymology; Candida rugosa; Cellulose - chemistry; Enzyme Activation; Enzyme Stability; Enzymes; Enzymes, Immobilized - analysis; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - ultrastructure; Eucalyptus - chemistry; immobilized enzymes; Infrared spectroscopy; Lignin - chemistry; Lipase - analysis; Lipase - chemistry; Membranes, Artificial; Polyethylene Glycols - chemistry; Retention; Studies; triacylglycerol lipase; Wood; Yeast

The objective of this work was to select an efficient methodology for preparing active samples of Candida rugosa lipase immobilized in wood cellulignin, to be applied in hydrolysis and ester reactions. For this purpose, lipase was immobilized in the matrix by physical adsorption (pure cellulignin) a...