MiR-452-5p mediates the proliferation, migration and invasion of hepatocellular carcinoma cells via targeting COLEC10

This study explored the potential function of miR-452-5p in hepatocellular carcinoma (HCC) and clarified the mechanism underlying HCC progression. Real-time quantitative PCR was used to detect miR-452-5p and COLEC10 mRNA expression in HCC, western blot was performed to test COLEC10 protein expressio...

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Published inPersonalized medicine Vol. 18; no. 2; p. 97
Main Authors Zheng, Jianxing, Cheng, Daming, Wu, Dongyang, Wang, Libing, Qu, Fengzhi, Wu, Xiaotang, Cheng, Ling, Wei, Yanbin, Liu, Xiaogang
Format Journal Article
LanguageEnglish
Published England 01.03.2021
Subjects
COLEC10
molecular mechanism
miR-452-5p
invasion
overexpression
proliferation
biological function
migration
oncogene
hepatocellular carcinoma
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Summary:This study explored the potential function of miR-452-5p in hepatocellular carcinoma (HCC) and clarified the mechanism underlying HCC progression. Real-time quantitative PCR was used to detect miR-452-5p and COLEC10 mRNA expression in HCC, western blot was performed to test COLEC10 protein expression. The regulatory mechanism of miR-452-5p/COLEC10 in HCC cells was explored using CCK-8, wound healing assay, Transwell and dual-luciferase reporter assay. MiR-452-5p was greatly upregulated in HCC cells, and it served as an oncogene playing an active role in HCC cell proliferation, migration and invasion. COLEC10 was identified as the target of miR-452-5p in HCC attenuating the promoting effect of miR-452-5p on HCC cells upon overexpression. MiR-452-5p can promote the progression of HCC via targeting COLEC10.
ISSN:1744-828X
DOI:10.2217/pme-2020-0027