Gene Expression Profiling of Systems Involved in the Metabolism and the Disposition of Xenobiotics: Comparison between Human Intestinal Biopsy Samples and Colon Cell Lines

• Published in: Drug metabolism and disposition Vol. 40; no. 4; pp. 694 - 705
• Main Authors: Bourgine, Joanna, Billaut-Laden, Ingrid, Happillon, Mélanie, Lo-Guidice, Jean-Marc, Maunoury, Vincent, Imbenotte, Michel, Broly, Franck
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2012
• Subjects: Adult; Aged; Biopsy; Cell Culture Techniques; Cell Line; Cluster Analysis; Colon - enzymology; Colon - metabolism; Colon - pathology; Female; Gene Expression; Gene Expression Profiling; Humans; Intestinal Mucosa - enzymology; Intestinal Mucosa - metabolism; Intestinal Mucosa - pathology; Male; Middle Aged; Oligonucleotide Array Sequence Analysis; Principal Component Analysis; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Tissue Distribution; Xenobiotics - metabolism; Xenobiotics - pharmacokinetics; XME; DMEM; ABC; TLDA; RT; PC; NR; P450; SLC; PCR; PCA

Intestinal cell lines are used as in vitro models for pharmacological and toxicological studies. However, a general report of the gene expression spectrum of proteins that are involved in the metabolism and the disposition of xenobiotics in these in vitro systems is not currently available. To fill...