Effect of PAI-1 Levels on the Molar Concentrations of Active Tissue Plasminogen Activator (t-PA) and t-PA/PAI-1 Complex in Plasma

• Published in: Blood Vol. 76; no. 5; pp. 930 - 937
• Main Authors: Chandler, Wayne L., Trimble, Sara L., Loo, Swee-Chin, Mornin, Dan
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01.09.1990; The Americain Society of Hematology
• Subjects: Antithrombins - metabolism; Biological and medical sciences; Cardiovascular system; Enzyme-Linked Immunosorbent Assay; Fibrinolysis; Humans; Kinetics; Macromolecular Substances; Medical sciences; Miscellaneous; Peptide Mapping; Pharmacology. Drug treatments; Plasminogen Inactivators - blood; Plasminogen Inactivators - isolation & purification; Substrate Specificity; Tissue Plasminogen Activator - blood; Tissue Plasminogen Activator - isolation & purification; Assay; Human; Treatment; Enzymatic activity; Enzyme; Heart disease; Plasminogen activator; Measurement method; Fibrinolysis

We determined the in vivo molar concentrations of active tissue plasminogen activator (t-PA), active plasminogen activator inhibitor type 1 (PAI-1), and t-PA/PAI-1 complex. t-PA activity was measured in plasma stabilized by immediate acidification. PAI-1 activity and t-PA/PAI-1 complex antigen were...