Improved sensitivity of the mouse interstitial cell testosterone assay with the addition of forskolin
Modification of the mouse interstitial cell testosterone assay by the addition of 1.5 microM forskolin to the incubation medium has improved the sensitivity of this luteinizing hormone bioassay from approximately 100 to 3 pg/tube of NIH rLH RP-2. Luteinizing hormone can be clearly detected in 1 micr...
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Published in | Canadian journal of physiology and pharmacology Vol. 70; no. 6; p. 866 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Canada
01.06.1992
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Abstract | Modification of the mouse interstitial cell testosterone assay by the addition of 1.5 microM forskolin to the incubation medium has improved the sensitivity of this luteinizing hormone bioassay from approximately 100 to 3 pg/tube of NIH rLH RP-2. Luteinizing hormone can be clearly detected in 1 microL of serum from rats castrated 1 week previously and 5 microL of serum from intact rats. Parallelism was noted between dilution curves of serum from intact and castrated rats, and the luteinizing hormone standard curve. Luteinizing hormone detected in serum samples from 30 intact rats by the improved bioassay and by radioimmunoassay was significantly correlated (r = 0.85). Secretion patterns of circulating luteinizing hormone in individual rats were similar when detected by either bioassay or radioimmunoassay. Thus, with the addition of forskolin, the mouse interstitial cell testosterone assay has been improved so that luteinizing hormone can be detected in small volumes of serum or plasma from male rats. |
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AbstractList | Modification of the mouse interstitial cell testosterone assay by the addition of 1.5 microM forskolin to the incubation medium has improved the sensitivity of this luteinizing hormone bioassay from approximately 100 to 3 pg/tube of NIH rLH RP-2. Luteinizing hormone can be clearly detected in 1 microL of serum from rats castrated 1 week previously and 5 microL of serum from intact rats. Parallelism was noted between dilution curves of serum from intact and castrated rats, and the luteinizing hormone standard curve. Luteinizing hormone detected in serum samples from 30 intact rats by the improved bioassay and by radioimmunoassay was significantly correlated (r = 0.85). Secretion patterns of circulating luteinizing hormone in individual rats were similar when detected by either bioassay or radioimmunoassay. Thus, with the addition of forskolin, the mouse interstitial cell testosterone assay has been improved so that luteinizing hormone can be detected in small volumes of serum or plasma from male rats. |
Author | Debertin, W J Pomerantz, D K |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/1423030$$D View this record in MEDLINE/PubMed |
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SubjectTerms | Animals Colforsin - pharmacology Extracellular Space - metabolism Luteinizing Hormone - blood Luteinizing Hormone - metabolism Male Mice Physiology - methods Radioimmunoassay Rats Sensitivity and Specificity Testis - cytology Testis - metabolism Testosterone - analysis Testosterone - biosynthesis |
Title | Improved sensitivity of the mouse interstitial cell testosterone assay with the addition of forskolin |
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