High Concentration Trypsin Assisted Fast In-Gel Digestion for Phosphoproteome Analysis

• Published in: Fēnxī huàxué Vol. 43; no. 10; pp. 1452 - 1458
• Main Authors: LIU, Fang-Jie, YE, Ming-Liang, PAN, Yan-Bo, ZOU, Han-Fa
• Format: Journal Article
• Language: English
• Published: 01.10.2015
• Subjects: Digestion; Enrichment; Mass spectrometry; Phosphorylation; Polyacrylamides; Proteins; Proteomics; Trypsin

Polyacrylamide gel electrophoresis (PAGE) is a powerful protein separation technology. Combined with mass spectrometry, it could identify thousands of proteins in proteomics analysis. However, the time-consuming procedure restricts its broad applications in proteomics study. In this work, it was fou...