New chromosome 21 DNA markers isolated by pulsed field gel electrophoresis from an ETS2-containing Down syndrome chromosomal region

• Published in: Human genetics Vol. 90; no. 4; p. 427
• Main Authors: Yaspo, M L, Crété, N, Chettouh, Z, Blouin, J L, Rahmani, Z, Stehelin, D, Sinet, P M, Créau-Goldberg, N, Delabar, J M
• Format: Journal Article
• Language: English
• Published: Germany 01.12.1992
• Subjects: Animals; Blotting, Southern; Chromosome Mapping; Chromosomes, Human, Pair 21; Cricetinae; DNA; Down Syndrome - genetics; Electrophoresis, Gel, Pulsed-Field; Genetic Markers; Humans; Hybrid Cells; Mice; Oncogenes; Polymorphism, Genetic; Retroviridae Proteins, Oncogenic - genetics
• DOI: 10.1007/BF00220472
• ISSN: 0340-6717

To generate new chromosome 21 markers in a region that is critical for the pathogenesis of Down syndrome (D21S55-MX1), we used pulsed field gel electrophoresis (PFGE) to isolate a 600-kb NruI DNA fragment from the WA17 hybrid cell line, which has retained chromosome 21 as the only human material. This fragment, which contains the oncogene ETS2, was used to construct a partial genomic library. Among the 14 unique sequences that were isolated, 3 were polymorphic markers and contained sequences that are conserved in mammals. Five of these markers mapped on the ETS2-containing NruI fragment and allowed us to define an 800-kb high-resolution PFGE map.