New chromosome 21 DNA markers isolated by pulsed field gel electrophoresis from an ETS2-containing Down syndrome chromosomal region

To generate new chromosome 21 markers in a region that is critical for the pathogenesis of Down syndrome (D21S55-MX1), we used pulsed field gel electrophoresis (PFGE) to isolate a 600-kb NruI DNA fragment from the WA17 hybrid cell line, which has retained chromosome 21 as the only human material. Th...

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Bibliographic Details
Published inHuman genetics Vol. 90; no. 4; p. 427
Main Authors Yaspo, M L, Crété, N, Chettouh, Z, Blouin, J L, Rahmani, Z, Stehelin, D, Sinet, P M, Créau-Goldberg, N, Delabar, J M
Format Journal Article
LanguageEnglish
Published Germany 01.12.1992
Subjects
Animals
Blotting, Southern
Chromosome Mapping
Chromosomes, Human, Pair 21
Cricetinae
DNA
Down Syndrome - genetics
Electrophoresis, Gel, Pulsed-Field
Genetic Markers
Humans
Hybrid Cells
Mice
Oncogenes
Polymorphism, Genetic
Retroviridae Proteins, Oncogenic - genetics
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Summary:To generate new chromosome 21 markers in a region that is critical for the pathogenesis of Down syndrome (D21S55-MX1), we used pulsed field gel electrophoresis (PFGE) to isolate a 600-kb NruI DNA fragment from the WA17 hybrid cell line, which has retained chromosome 21 as the only human material. This fragment, which contains the oncogene ETS2, was used to construct a partial genomic library. Among the 14 unique sequences that were isolated, 3 were polymorphic markers and contained sequences that are conserved in mammals. Five of these markers mapped on the ETS2-containing NruI fragment and allowed us to define an 800-kb high-resolution PFGE map.
ISSN:0340-6717
DOI:10.1007/BF00220472