Aberrant, canavanyl protein formation and the ability to tolerate or utilize L-canavanine

L-Canavanine, 2-amino-4-(guanidinooxy)butyric acid, and L-arginine incorporation into de novo synthesized proteins was compared in six organisms. Utilizing L-[guanidinooxy14C]canavanine and L-[guanidino14C]arginine at substrate saturation, the canavanine to arginine incorporation ratio was determine...

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Bibliographic Details
Published inExperientia Vol. 43; no. 5; p. 558
Main Authors Rosenthal, G A, Berge, M A, Bleiler, J A, Rudd, T P
Format Journal Article
LanguageEnglish
Published Switzerland 15.05.1987
Subjects
Animals
Arginine - metabolism
Canavanine - metabolism
Carbon Radioisotopes
Insecta - metabolism
Larva
Lepidoptera - metabolism
Moths - metabolism
Plants - metabolism
Species Specificity
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Summary:L-Canavanine, 2-amino-4-(guanidinooxy)butyric acid, and L-arginine incorporation into de novo synthesized proteins was compared in six organisms. Utilizing L-[guanidinooxy14C]canavanine and L-[guanidino14C]arginine at substrate saturation, the canavanine to arginine incorporation ratio was determined in de novo synthesized proteins. Caryedes brasiliensis and Sternechus tuberculatus, canavanine utilizing insects; Canavalia ensiformis, a canavanine storing plant; and to a lesser extent Heliothis virescens, a canavanine resistant insect, failed to accumulate significant canavanyl proteins. By contrast, Manduca sexta, a canavanine-sensitive insect, and Glycine max, a canavanine free plant, readily incorporated canavanine into newly synthesized proteins. This study supports the contention that the incorporation of canavanine into proteins in place of arginine contributes significantly to canavanine's antimetabolic properties.
ISSN:0014-4754
DOI:10.1007/BF02143585