Using tungsten oxide quantum-dot enhanced electrochemiluminescence to measure thrombin activity and screen its inhibitors
A thrombin-activity-based electrochemiluminescence (ECL) biosensor was successfully constructed using tungsten oxide quantum dots (WO3-x QDS) as the co-reactant and thrombin-cleavable peptides as the recognizer. Specifically, Ru(bpy)32+ were doped on silica nanoparticles (Ru@SiO2), which greatly enh...
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Published in | Talanta (Oxford) Vol. 267; p. 125267 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
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Elsevier B.V
15.01.2024
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Abstract | A thrombin-activity-based electrochemiluminescence (ECL) biosensor was successfully constructed using tungsten oxide quantum dots (WO3-x QDS) as the co-reactant and thrombin-cleavable peptides as the recognizer. Specifically, Ru(bpy)32+ were doped on silica nanoparticles (Ru@SiO2), which greatly enhanced the ECL potential. AuNPs@WO3-x QDs composite was then prepared to accelerate electron transfer and improve the ECL signal by 219 times. Under ideal conditions, the limit of detection for thrombin in serum was determined to be 0.28 μU/mL with a linear range from 1 μU/mL to 1 U/mL. In addition, the developed ECL biosensor was used to screen for thrombin inhibitors from 12 compounds in Artemisiae Argyi Folium. Among the compounds tested, it was observed that 100 μmol/L luteolin exhibited a significantly higher inhibition rate (exceeding 80%) compared to apigenin, isorhamnetin, naringin, or eriodictyol. In an in-vitro anticoagulation experiment, luteolin (100 μmol/L) prolonged APTT by 49%, and the molecular docking assay indicated that luteolin had binding sites of Gly219 and Asp189 in the active pockets of thrombin. This may have been the main reason underpinning luteolin's anticoagulation effects. Overall, the Ru@WO3-x QDS ECL biosensor provided a reliable strategy for thrombin activity assay and screening of anticoagulant agents.
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•The composite material, AuNPs@WO3-x QDs, significantly enhances electron transfer, resulting in a remarkable 219-fold improvement in the ECL signal.•The limit of detection for thrombin activity in serum was successfully determined to be 0.28 μU/mL.•Luteolin displayed an outstanding inhibition rate surpassing 80% and has been identified as a promising anti-thrombin inhibitor during the screening process. |
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AbstractList | A thrombin-activity-based electrochemiluminescence (ECL) biosensor was successfully constructed using tungsten oxide quantum dots (WO3-x QDS) as the co-reactant and thrombin-cleavable peptides as the recognizer. Specifically, Ru(bpy)32+ were doped on silica nanoparticles (Ru@SiO2), which greatly enhanced the ECL potential. AuNPs@WO3-x QDs composite was then prepared to accelerate electron transfer and improve the ECL signal by 219 times. Under ideal conditions, the limit of detection for thrombin in serum was determined to be 0.28 μU/mL with a linear range from 1 μU/mL to 1 U/mL. In addition, the developed ECL biosensor was used to screen for thrombin inhibitors from 12 compounds in Artemisiae Argyi Folium. Among the compounds tested, it was observed that 100 μmol/L luteolin exhibited a significantly higher inhibition rate (exceeding 80%) compared to apigenin, isorhamnetin, naringin, or eriodictyol. In an in-vitro anticoagulation experiment, luteolin (100 μmol/L) prolonged APTT by 49%, and the molecular docking assay indicated that luteolin had binding sites of Gly219 and Asp189 in the active pockets of thrombin. This may have been the main reason underpinning luteolin's anticoagulation effects. Overall, the Ru@WO3-x QDS ECL biosensor provided a reliable strategy for thrombin activity assay and screening of anticoagulant agents. A thrombin-activity-based electrochemiluminescence (ECL) biosensor was successfully constructed using tungsten oxide quantum dots (WO3-x QDS) as the co-reactant and thrombin-cleavable peptides as the recognizer. Specifically, Ru(bpy)32+ were doped on silica nanoparticles (Ru@SiO2), which greatly enhanced the ECL potential. AuNPs@WO3-x QDs composite was then prepared to accelerate electron transfer and improve the ECL signal by 219 times. Under ideal conditions, the limit of detection for thrombin in serum was determined to be 0.28 μU/mL with a linear range from 1 μU/mL to 1 U/mL. In addition, the developed ECL biosensor was used to screen for thrombin inhibitors from 12 compounds in Artemisiae Argyi Folium. Among the compounds tested, it was observed that 100 μmol/L luteolin exhibited a significantly higher inhibition rate (exceeding 80%) compared to apigenin, isorhamnetin, naringin, or eriodictyol. In an in-vitro anticoagulation experiment, luteolin (100 μmol/L) prolonged APTT by 49%, and the molecular docking assay indicated that luteolin had binding sites of Gly219 and Asp189 in the active pockets of thrombin. This may have been the main reason underpinning luteolin's anticoagulation effects. Overall, the Ru@WO3-x QDS ECL biosensor provided a reliable strategy for thrombin activity assay and screening of anticoagulant agents. [Display omitted] •The composite material, AuNPs@WO3-x QDs, significantly enhances electron transfer, resulting in a remarkable 219-fold improvement in the ECL signal.•The limit of detection for thrombin activity in serum was successfully determined to be 0.28 μU/mL.•Luteolin displayed an outstanding inhibition rate surpassing 80% and has been identified as a promising anti-thrombin inhibitor during the screening process. |
ArticleNumber | 125267 |
Author | Zhang, Jing Qiao, Yanru Wang, Sicen Deng, Zijie Xu, Dan Zhang, Junbo Guo, Dongnan Li, You Tan, Xueping Hou, Xiaofang |
Author_xml | – sequence: 1 givenname: Jing surname: Zhang fullname: Zhang, Jing organization: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China – sequence: 2 givenname: Dan orcidid: 0000-0003-0548-3833 surname: Xu fullname: Xu, Dan organization: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China – sequence: 3 givenname: Zijie surname: Deng fullname: Deng, Zijie organization: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China – sequence: 4 givenname: Xueping surname: Tan fullname: Tan, Xueping organization: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China – sequence: 5 givenname: Dongnan orcidid: 0000-0001-6599-047X surname: Guo fullname: Guo, Dongnan organization: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China – sequence: 6 givenname: Yanru orcidid: 0000-0001-9715-9031 surname: Qiao fullname: Qiao, Yanru organization: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China – sequence: 7 givenname: You surname: Li fullname: Li, You organization: Department of Peripheral Vascular Disease, The First Affiliated Hospital of the Medical College of Xi'an Jiaotong University, Xi'an 710061, China – sequence: 8 givenname: Xiaofang orcidid: 0000-0002-8558-4521 surname: Hou fullname: Hou, Xiaofang email: houxiaofang@mail.xjtu.edu.cn organization: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China – sequence: 9 givenname: Sicen surname: Wang fullname: Wang, Sicen email: wangsc@mail.xjtu.edu.cn organization: School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an 710061, China – sequence: 10 givenname: Junbo surname: Zhang fullname: Zhang, Junbo email: zhangjunbo@xjtufh.edu.cn organization: Department of Peripheral Vascular Disease, The First Affiliated Hospital of the Medical College of Xi'an Jiaotong University, Xi'an 710061, China |
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Keywords | Electrochemiluminescence biosensors Anti-Thrombin inhibitors Tungsten oxide quantum dots Thrombin activity Tris(2,2′-bipyridine)-Ruthenium(II) |
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Snippet | A thrombin-activity-based electrochemiluminescence (ECL) biosensor was successfully constructed using tungsten oxide quantum dots (WO3-x QDS) as the... |
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SubjectTerms | Anti-Thrombin inhibitors Electrochemiluminescence biosensors Thrombin activity Tris(2,2′-bipyridine)-Ruthenium(II) Tungsten oxide quantum dots |
Title | Using tungsten oxide quantum-dot enhanced electrochemiluminescence to measure thrombin activity and screen its inhibitors |
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