Immunosequencing identifies signatures of cytomegalovirus exposure history and HLA-mediated effects on the T cell repertoire

• Published in: Nature genetics Vol. 49; no. 5; pp. 659 - 665
• Main Authors: Emerson, Ryan O, DeWitt, William S, Vignali, Marissa, Gravley, Jenna, Hu, Joyce K, Osborne, Edward J, Desmarais, Cindy, Klinger, Mark, Carlson, Christopher S, Hansen, John A, Rieder, Mark, Robins, Harlan S
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.05.2017; Nature Publishing Group
• Subjects: 45; 45/23; 631/114; 631/208/248; 631/208/514/1948; 631/250; Agriculture; Algorithms; Animal Genetics and Genomics; Antigens; Biomedicine; Cancer Research; Coding; Cohort Studies; Cytomegalovirus; Cytomegalovirus - genetics; Cytomegalovirus - immunology; Cytomegalovirus - physiology; Cytotoxicity; Diagnostic systems; Epitopes, T-Lymphocyte - genetics; Epitopes, T-Lymphocyte - immunology; Exposure; Gene Function; High-Throughput Nucleotide Sequencing - methods; Histocompatibility Testing - methods; HLA Antigens - genetics; HLA Antigens - immunology; HLA-A Antigens - genetics; HLA-A Antigens - immunology; HLA-B Antigens - genetics; HLA-B Antigens - immunology; Host-Pathogen Interactions - immunology; Human Genetics; Humans; Immunology; In vitro methods and tests; Infections; Lymphocytes; Lymphocytes T; Medical research; Models, Immunological; Pathogens; Receptors; Receptors, Antigen, T-Cell - genetics; Receptors, Antigen, T-Cell - immunology; Reproducibility of Results; Signatures; T cell receptors; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; T-Lymphocytes - virology; Viral infections

Ryan Emerson and colleagues report immunosequencing of the variable region of the TCRβ chain in 666 individuals with known cytomegalovirus (CMV) status. They show that CMV status and HLA genotype shape the T cell repertoire and demonstrate proof of principle that TCRβ sequencing can be used as a spe...