Characterization of Phages YuuY, KaiHaiDragon, and OneinaGillian Isolated from Microbacterium foliorum

Microbacterium foliorum is a Gram-positive bacteria found in organic matter. Three lytic bacteriophages, KaiHaiDragon, OneinaGillian, and YuuY, were isolated from M. foliorum strain NRRL B-24224. Phage YuuY in particular expresses a broad host range as it possesses the ability to infect closely rela...

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Bibliographic Details
Published inInternational journal of molecular sciences Vol. 23; no. 12; p. 6609
Main Authors Kim, Uylae, Paul, Elizabeth S., Diaz, Arturo
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 14.06.2022
MDPI
Subjects
Adsorption
Amino acids
Bacteria
bacteriophages
Bioinformatics
Burst size
Carbohydrates
Dairy products
Genes
Genomes
genomics
Gram-positive bacteria
Host range
Infections
Latent period
Microbacterium
Morphology
Organic matter
phage therapy
Phages
Physical characteristics
Science education
Sodium
Temperature tolerance
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Summary:Microbacterium foliorum is a Gram-positive bacteria found in organic matter. Three lytic bacteriophages, KaiHaiDragon, OneinaGillian, and YuuY, were isolated from M. foliorum strain NRRL B-24224. Phage YuuY in particular expresses a broad host range as it possesses the ability to infect closely related bacterial species Microbacterium aerolatum at a high plating efficiency. Characterization tests were performed on all three Microbacterium phage to assess morphology, genomic characteristics, pH and thermal stabilities, life cycle, and the type of receptor used for infection. All three phages showed similar pH stability, ranging from pH 5–11, except for KaiHaiDragon, which had a reduced infection effectiveness at a pH of 11. YuuY possessed a significantly higher temperature tolerance compared to the other Microbacterium phages as some phage particles remained viable after incubation temperatures of up to 80 °C. Based on the one-step growth curve assay, all three Microbacterium phages possessed a relatively short latent period of 90 min and an approximately two-fold burst size factor. Moreover, all three phages utilize a carbohydrate receptor to initiate infection. Based on bioinformatics analysis, YuuY, KaiHaiDragon and OneinaGillian were assigned to clusters EA10, EC, and EG, respectively.
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ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms23126609