Neuron/Oligodendrocyte Myelination Coculture

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1791; p. 131
• Main Authors: Pang, Yi, Simpson, Kimberly, Miguel-Hidalgo, José Javier, Savich, Renate
• Format: Journal Article
• Language: English
• Published: United States 2018
• Subjects: Animals; Axons - metabolism; Axons - ultrastructure; Biomarkers; Cell Culture Techniques; Cell Lineage; Coculture Techniques; Fluorescent Antibody Technique; Immunohistochemistry; Mice; Myelin Sheath - metabolism; Myelin Sheath - ultrastructure; Neural Stem Cells; Neuroglia - cytology; Neuroglia - metabolism; Neurons - cytology; Neurons - metabolism; Oligodendroglia - cytology; Oligodendroglia - metabolism; Primary Cell Culture; Rabbits; Rats; Spinal Cord - cytology; Spinal Cord - metabolism; Embryonic; Cell culture; Axon; Myelination; Spinal cord; Oligodendrocyte

Myelination cell culture systems are useful tools for studying myelin biology and myelin-related disorders. Compared to a number of established protocols for dissociated pure oligodendrocyte (OL) culture, methods for myelination culture are limited. We recently developed a mixed neuron-glia cocultur...