Telomeric repeat-containing RNA increases in aged human cells

• Published in: Nucleic acids research Vol. 53; no. 13
• Main Authors: Hsieh, Yu-Hung, Tai, Chin-Hua, Yeh, Meng-Ting, Chen, Yu-Chen, Yang, Po-Cheng, Yen, Chien-Ping, Shen, Hong-Jhih, Yeh, Chan-Hsien, Kuo, Hung-Chih, Han, Der-Sheng, Chu, Hsueh-Ping Catherine
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 08.07.2025
• Subjects: Aged; Aging - genetics; Alzheimer Disease - genetics; Alzheimer Disease - metabolism; Alzheimer Disease - pathology; Brain - metabolism; CCCTC-Binding Factor - genetics; Cell Differentiation - genetics; Cellular Senescence - genetics; Fibroblasts - metabolism; Genomics; Histones - metabolism; Humans; Promoter Regions, Genetic; Repetitive Sequences, Nucleic Acid; RNA - genetics; RNA-Seq; Single-Cell Analysis; Telomere - genetics; Transcription, Genetic

Telomeric repeat-containing RNA (TERRA), transcribed from subtelomeric regions toward telomeric ends, poses challenges in deciphering its complete sequences. Utilizing TERRA-capture RNA-seq and Oxford Nanopore direct RNA sequencing to acquire full-length TERRA, we annotate TERRA transcription region...