Sensitivity evaluation of a modified real-time reverse-transcription PCR primer to detect a measles virus variant in Japan in 2024

• Published in: Japanese Journal of Infectious Diseases p. JJID.2024.304
• Main Authors: Morikawa, Saeko, Motomura, Kazushi, Kanbayashi, Daiki, Hiroi, Satoshi, Koyama, Mei, Otsuki, Noriyuki, Kaida, Atsushi, Miyama, Takeshi, Kurata, Takako, Hirai, Yuki
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases 28.02.2025
• Subjects: Measles; Real-time PCR; Sensitivity; Variant; Variant; Sensitivity; Real-time PCR; Measles

A measles outbreak occurred in Japan in February 2024 due to a measles virus variant that was imported from central Asian countries with three mismatches at the PCR reverse primer (MVN1213R) annealing site. To examine and improve the impact of real-time PCR effectiveness for detecting this variant,...